Influence of transarterial chemoembolization on angiogenesis and expression of vascular endothelial growth factor and basic fibroblast growth factor in rat with Walker-256 transplanted hepatoma: An experimental study

Table 1 Drug administration in the three groups

Group	Drug administration
Control	0.3 mL normal saline
Arterial infusion (AI group)	5-fluorouracil (5-FU) 20 mg/kg
Transarterial chemoembolization (TACE group)	lipiodol ultra-fluis (Andre Guerbet Laboratories, Aulnay-Sous-Bios, France) 0.5 mL/kg and 5-FU 20 mg/kg

Table 2 Tumor volumes and tumor growth rate before and after therapy (¯x ± s)

Group	Tumor volume (mm3)		Tumor growth rate (%)
	Before therapy	2 wk after therapy
Control	195.6 ± 65.9	963.6 ± 214.8	459.2 ± 52.7
AI	218.9 ± 48.7	868.9 ± 179.8	412.2 ± 18.9
TACE	210.2 ± 59.2	356.5 ± 78.4	173.4 ± 20.4
F value	0.272	37.818	197.932
P value	0.764	< 0.001	< 0.001

Table 3 MVD counts, expression of VEGF and b-FGF in each group

Group	n	MVD (¯x ± s)	VEGF					b-FGF
			0	+	++	+++	Mean	0
Control	20	80.80 ± 20.94	5	8	5	2	1.2	14	6
AI	20	83.55 ± 18.26	5	7	6	2	1.25	15	5
TACE	20	85.20 ± 23.91	3	9	5	3	1.4	14	6
Statistic value		F = 0.193			χ² = 0.449			χ² = 0.141
P value		P = 0.873			P = 0.799			P = 0.922

Table 4 MVD count in negative and positive VEGF groups after TACE

Expression of VEGF	n	MVD (per high power field) (¯x ± s)
Negative group	13	67.86 ± 11.66
Positive group	47	87.36 ± 22.97
T value		2.543
P value		0.014