Relationship between overexpression of NK-1R, NK-2R and intestinal mucosal damage in acute necrotizing pancreatitis

Table 1 The sequence of primers used for RT-PCR

Primers	Sequence	Primer size (bp)	PCR products size (bp)
NK-1R
Forward primer	5'-CAT CAA CCC AGA TCT CTA CC-3'	20	380
Reverse primer	5'-GCT GGA GCT TTC TGT CAT GGA-3'	21
NK-2R
Forward primer	5'-CAT CAC TGT GGA CGA GGG GG-3'	20	491
Reverse primer	5'-TGT CTT CCT CAG TTG GTG TC-3'	20
GAPDH
Forward primer	5'-TGA AGG TCG GTG TCA ACG GAT TTG GC-3'	26	999
Reverse primer	5'-CAT GTA GGC CAT GAG GTC CAC CAC-3'	24

Table 2 The pathological score of intestinal mucosa

Groups	0 h	6 h	12 h	24 h	48 h
Control	0.92 ± 0.47	0.90 ± 0.35	0.93 ± 0.38	0.93 ± 0.29	1.07 ± 0.36
ANP		2.11 ± 0.47a	2.65 ± 0.49b	3.91 ± 0.82b	4.89 ± 1.21b

^aP < 0.05 vs each time point of control group, respectively;

^bP < 0.01 vs each time point of control group, respectively

Table 3 Changes of intestinal mucosal permeability

Groups	0 h	6 h	12 h	24 h	48 h
Control	0.0411 ± 0.0156	0.0455 ± 0.0174	0.0532 ± 0.0188	0.0693 ± 0.0153	0.0698 ± 0.0223
ANP		0.2367 ± 0.1132a	0.3457 ± 0.0473a	0.6651 ± 0.1411a	0.7021 ± 0.1523a

^aP < 0.01 vs each time point of control group, respectively