Expression of vascular endothelial growth factor and its receptors KDR and Flt-1 in gastric cancer cells

Table 1 Gene specific primers for PCR

gene	GenBank	Orientation Accession Number	Sequence
VEGF	AF022375	Forward	5’-GGGGGATCCGCCTCCGAAACCATGAACTT-3’
		Reverse	5’-CCCGAATTCTCCTGGTGAGAGATCTGGTT-3
Flt-1	AF063657	Forward-1	5’-TCTAGGATCCATGGTCAGCTACTGGGACACC-3’
		Forward-2	5'-AAGGGATCCCCTGAACTGAGTTTAAAA-3'
		Reverse	5’-GGCGAATTCTGGGGTTTCACATTGAC-3
KDR	AF063658	Forward	5’-TAAGGATCCCACTCAAACGCTGAC-3’
			5’-GGAGAATTCTCAACTGCATGCCTGGCAG-3’
			5’-TCCTGGGCACCTTCTA-3’
GAPDH	AF261085	Forward	5'-ACCACAGTCCATGCCATCAC-3'
			5'-TCCACCACCCTGTTGCTGTA-3'

(In bold sequences are restriction endonuclease recogni-tion sites, which were added for further cloning).

Table 2 Expression of Flt-1 and KDR on gastric carcinoma specimens

Expression of Flt-1 and KDR	n	Percentage of 24 cases
Flt-1(+) KDR(+)	15	62.5
Flt-1(-) KDR(-)	5	20.8
Flt-1(+) KDR(-)	4	16.7
Flt-1(-) KDR(+)	0	0