Methodologic research on TIMP-1, TIMP-2 detection as a new diagnostic index for hepatic fibrosis and its significance

Table 1 Relationship between coated McAb and the sensitivity of SPASE

Coated McAb (mg•L⁻¹)	Serum titer							Negative control
	1:1	1:10	1:20	1:50	1:100	1:200	1:500
12.5	+++	++	+	-	-	-	-	-
25	++++	+++	++	+	-	-	-	-
50	++++	++++	++++	++++	++	+	-	-
100	++++	++++	++++	+++	++	+	-	-
200	++++	+++	+++	++	+	-	-	-
400	+++	++	++	++	+	-	-	-

Table 2 Relation between the density of sensitized erythrocytes and the sensitivity of SPASE

Density of sensitized erythrocytes	Serum titer							Negative control
	1:1	1:10	1:20	1:50	1:100	1:200	1:500
1.5•L⁻¹	+++	++	++	-	-	-	-	-
2.5•L⁻¹	++++	++++	++++	+++	++	+	-	-
3.0•L⁻¹	++++	++++	+++	++	+	+	-	-
6.0•L⁻¹	+++	+++	++	+	-	-	-	-
10.0•L⁻¹	++	+++	++	-	-	-	-	-

Table 3 Repetitive experiment of samples

	Well numbers	(¯x ± s)	CV (%)
In the same lot	15	0.642 ± 0.0389	6.06
In different lot	5	0.575 ± 0.044	7.65

Table 4 Results of detecting TIMP-1 and TIMP-2 in different sera

Clinical type	n	TIMP-1		TIMP-2
		Positive number	%	Positive number	%
Acute hepatitis	128	21	16.4	16	12.6
Chronic hepatitis	174	59	33.9	48	27.6
Hepatic cirrhosis	106	78	73.6	65	61.3

Table 5 Expressing of TIMP-1 and TIMP-2 in liver

Group	n	TIMP-1				TIMP-2
		-	+	++	+++	-	+	++	+++
Normal group	10	10	0	0	0	10	0	0	0
Hepatic cirrhosis	40	0	8	4	28	0	8	10	22

Table 6 Expressing of TIMP-1 mRNA and TIMP-2 mRNA in liver of hepatic cirrhosis

Group	n	TIMP-1				TIMP-2
		+++	++	+	-	+++	++	+	-
Hepatic cirrhosis	40	32	6	2	0	22	16	2	0
Normal liver	10	0	0	0	10	0	0	0	10