Impact of GFRA1 gene reactivation by DNA demethylation on prognosis of patients with metastatic colon cancer

Table 1 Comparison of GFRA1 demethylation-high proportion by MethyLight for colon carcinoma tissues from 144 patients with various clinicopathological characteristics

		dmGFRA11-high cases	dmGFRA1-low cases	dmGFRA1-high proportion (%)	P value1
Age (yr)	< 60	35	25	58.3	0.091
	≥ 60	37	47	44.0
Sex	Male	42	46	47.7	0.494
	Female	30	26	53.6
Location	Sigmoid	33	40	45.2	0.243
	Others	39	32	54.9
Differentiation	Poor	12	1	92.3	0.0022
	Moderate/well	62	69	45.8
Vascular embolus	Negative	58	65	47.2	0.098
	Positive	14	7	66.7
pTNM stage	I/II	33	39	45.8	0.314
	III/IV	38	32	54.3
Local invasion	T1-2	5	6	45.5
	T3	44	27	62.0	0.0043
	T4	22	38	36.7
Lymphatic metastasis	N0	32	41	43.8	0.134
	N1-3	40	31	56.3
Distant metastasis	M0	58	68	46.0	0.0212
	M1	14	4	77.8

¹Chi-square test;

²Fisher's exact test;

³T3 vs T4; dmGFRA1: Demethylation GFRA1.

Table 2 Results of multivariate analysis for survival factors in colon cancer patients

	All patients (n = 144)		Patients with metastatic CC (n = 89)
	Adjusted HR (95%CI)	P value	Adjusted HR (95%CI)	P value
Low dmGFRA1	0.49 (0.24-0.98)	0.044	0.41 (0.18-0.91)	0.029
Age	1.68 (0.83-3.40)	0.147	1.69 (0.77-3.73)	0.191
Sex	1.45 (0.76-2.76)	0.256	1.44 (0.71-2.91)	0.317
Location	2.60 (1.32-5.14)	0.006	3.22 (1.48-7.01)	0.003
Differentiation	1.95 (0.65-5.89)	0.235	2.02 (0.62-6.61)	0.246
Vascular embolus	0.32 (0.15-0.68)	0.003	0.33 (0.15-0.74)	0.007
Local invasion	1.27 (0.71-2.28)	0.421	1.16 (0.60-2.24)	0.668
Metastasis	4.92 (2.04-11.84)	0.000

CC: Colon cancer; HR: Hazard ratio; CI: Confidence interval.