Basic Study
Copyright ©The Author(s) 2020.
World J Gastroenterol. May 14, 2020; 26(18): 2203-2220
Published online May 14, 2020. doi: 10.3748/wjg.v26.i18.2203
Table 1 Primer sequences for real-time quantitative polymerase chain reaction
Gene nameForward sequenceReverse sequence
TNF-αTGCCTCAGCCTCTTCTCATTGAGCCCATTTGGGAACTTCT
IL-6AGTTGCCTTCTTGGGACTGACCTCCGACTTGTGAAGTGGT
IL-1βGAAGTCAAGACCAAAGTGGTGAAGTCAACTATGTCCCG
CCR2CACCGTATGACTATGATGATGCAGGAGAGCAGGTCAGAGAT
p47phoxGCCCAAAGATGGCAAGAATAATGACCTCAATGGCTTCACC
p67phoxAGCAGAAGAGCAGTTAGCATTGGTGCTTTCCATGGCCTTGTC
p22phoxGTAGATGCCGCTCGCAATGGCCAGATGGGGCAGATCGAGTGGGCCATGT
gp91phoxCTGAGCGAATTGTACGTGCTTATCACAGCCACAAGC
αSMATGTGCTATGTCGCTCTGGACCCAATGAAAGATGGCTG GAA
TGFβ1ATTCCTGGCGTTACCTTGGAGCCCTGTATTCCGTCTCCT
Col1a1TGTTCAGCTTTGTGGACCTCAGCTGACTTCAGGGATGT
Col2a1ACCTCAGGGTGTTCAAGGTGCGGATTCCAATAGGACCAGA
Col3a1GGTGGCTTTCAGTTCAGCTATGGTCTTGCTCCATTCACCAGTGT
MAT1ACAATGTGCTCGTGGCTCTGGAGTCCTCTGTCTCGTCAGTGGCATAG
ALDH1L1GCACGGCTCCATCATCTACCATCGTCATCTGGAAGCACCTCACACTC
SREBP1cCCAGCCTTTGAGGATAACCATGCAGGTCAGACACAGGAAG
SCDAGCTGGTGATGTTCCAGAGGCAAGAAGGTGCTGACGAACA
ACACAGAATATCCAGATGGCCGAGACCTTCTGCTCTGGCAAGTTC
FASNGCCTAACACCTCTGTGCAGTGGCAATACCCGTTCCCTGAA
PPARγACAAGAGCTGACCCAATGGTGGCTCTTCATGTGGCCTGTT
ACADLACTCCGCCTCCGCTTCCATGTACCACCGTAGATCGGCTGAACTC
FABP1GTCTGCCTGAGGACCTCATCCAGTCATGGTCTCCAGTTCGCACTCC
CPT1αCCACGAAGCCCTCAAACAGACACACCCACCACCACGATAA
FATP2CACGACAGAGTTGGAGACACCTTCCCGATGCGACCTTCATGACCTG
Table 2 Serological lipid metabolism indexes in each group
ControlHFDFolic acid
ALT (U/L)38.50 ± 1.58134.0 ± 8.02b82.13 ± 7.19d
AST (U/L)88.00 ± 4.39225.4 ± 10.57b176.3 ± 15.3d
FBG (mmol/L)10.45 ± 0.6613.33 ± 0.40b7.83 ± 0.30d
TG (mmol/L)0.57 ± 0.040.76 ± 0.04b0.44 ± 0.02d
TC (mmol/L)1.13 ± 0.042.26 ± 0.12b1.48 ± 0.04d
HDL (mmol/L)0.94 ± 0.030.75 ± 0.05b0.74 ± 0.04
LDL (mmol/L)0.24 ± 0.021.37 ± 0.10b0.89 ± 0.03d
TBA (µmol/L)37.5 ± 5.5768 ± 7.49b44.17 ± 3.92c
Hcy (µmol/L)7.35 ± 0.2913.05 ± 0.52b11.17 ± 0.42d