Screening of aptamers and their potential application in targeted diagnosis and therapy of liver cancer

Table 1 Current literature summary of aptamers for in vitro detection of liver cancer

First author	Year	Aptamer	Target	System for detection	Method for detection
Hu et al[51]	2017	TLS11a	HepG2 cells	Bio-TLS11a and SA-FSNPs	Fluorescence Microscopy
Wang et al[52]	2016	sLex-AP	CTCs	CTC-BioT Chip	Fluorescence Microscopy
Joo et al[53]	2017	AFB1-Apt	AFB1	FAM modified AFB1-Apt and GO	Fluorescence Microscopy
Kaur et al[54]	2019	SL2-B	HepG2 cells	QDs labeled SL2-B	Fluorescence Microscopy
Zhou et al[55]	2019	Slow-off aptamer (TD10); fast-off aptamer	DKK1 protein	5′ amino C6 modified TD10-based ELISA; 5′ FAM labeled fast-off aptamer	ELISA (TD10); dot blot and flow cytometry (fast-off aptamer)

Table 2 Current literature summary of aptamers for in vivo imaging of liver cancer

First author	Year	Aptamer	Type of tumor	Probe for detection	Method for detection
Yan et al[59]	2018	CD105-Apt	Tiny HCC	Conjugated CD105-Apt to paramagnetic and fluorophore on the G5 dendrimer	MRI
Zhong et al[60]	2019	mEND	HCC	mEND-Fe O @CMCS	MRI
Zhao et al[61]	2018	AP613-1	HCC expressing GPC3	Apt-USPIO	MRI
Babaei et al[69]	2017	EpCAM-Apt	HCC	EpCAM-PEG-Au@Si-5-FU NPs	CT

Apt: Aptamer; HCC: Hepatocellular carcinoma; MRI: Magnetic resonance imaging; CT: Computed tomography; USPIO: Ultrasmall superparamagnetic iron oxide.

Table 3 Current literature summary on the application of aptamers in targeted therapy for liver cancer

First author	Year	Aptamer	Target	System for therapy	Function
Hu et al[66]	2018	TLS11a	H22 cells	TLS11a/CD3	Immunity therapy
Xiao et al[67]	2017	EpDT3	EpCAM-positive cells HepG2	EpDT3-PEG-Ad5-PTEN (EPAP)	Gene therapy
Babaei et al[69]	2017	EpCAM-Apt	EpCAM-positive cells HepG2	EpCAM-PEG-Au@Si-5-FU NPs	Chemotherapy
Lan et al[70]	2019	TLS11a	HepG2 cells	Apt-BMSF@Pt	Phototherapy
Liu et al[72]	2016	ST21	SK-Hep-1 cells	FasudilST21-H3R5-PEG miR195	Chemotherapy and gene therapy
Cheng et al[73]	2019	TLS1c	MEAR cells	BioTL Cab/lipo	Chemotherapy