Association between TLR7 copy number variations and hepatitis B virus infection outcome in Chinese

Table 1 Forward and reverse primers of TLR7 target segments

Probe	Chromosome	Location (ref 37 database)1	Amplification length (sample, competitive)2	Primer binding region 1	Primer binding region 2
TLR7-2	ChrX	12885611-12885868	285 (+0, -2)	GGGCCCATCTCAAGCTGATCT	TAATGAAGGGGGCATGTCACAA
TLR7-3	ChrX	12905987-12906102	143 (+0, -2)	GCACCTGTGATGCTGTGTGGTT	AGCACACAAGGGCCAAAGTGTG

¹GRCH37 primary reference assembly;

²Sample: Sample DNA; competitive: Competitive DNA.

Table 2 Baseline characteristics of study patients

	n	Males/females	Age (yr)	95%CI
AHB	300	165/135	45.26 ± 15.54	43.94-47.03
CHB	253	198/55	33.98 ± 12.07	32.49-35.48
LC	248	196/52	50.26 ± 13.29	48.60-51.92
HCC	122	101/21	53.72 ± 12.50	51.48-55.96

Comparison of age among groups: F = 83.216, P < 0.01; comparison of sex among groups: χ² = 60.296, P < 0.01. AHB: Acute hepatitis B virus infection; CHB: Chronic hepatitis B virus infection; LC: Liver cirrhosis; HCC: Hepatocellular carcinoma.

Table 3 Estimated risk of TLR7 copy number in the development of chronic hepatitis B virus infection

Gender	CN	Chronic group	Acute group	OR	95%CI:	P value
Male	< 1	337	68	0.33	0.23-0.47	< 0.001
	> 1	158	97
Female	< 2	96	63	0.29	0.17-0.49	< 0.001
	> 2	32	72

CN: Copy number.

Table 4 Frequency distribution of TLR7 copy number in chronic hepatitis B virus infection groups

Gender	CN	CHB	LC	HCC	χ2	P value
Male	< 1	137	137	63	1.923	0.382
	> 1	61	59	38
Female	< 2	39	42	15	1.557	0.459
	> 2	16	10	6

CN: Copy number; CHB: Chronic hepatitis B virus infection; LC: Liver cirrhosis; HCC: Hepatocellular carcinoma.