Dachaihu decoction ameliorates pancreatic fibrosis by inhibiting macrophage infiltration in chronic pancreatitis

Table 1 Contents of Dachaihu decoction

Chinese name	Botanical name	Common name	Genus	Family	Weight, g	Part used
Chai hu	Bupleurumabchasicum Manden	Radix bupleuri	Bupleurum	umbelliferae	15	Root
Huang qin	Scutellaria baicalensis Georgi baicalensis	Radix scutellariae	Scutellaria	Labiatae	9	Root
Zhishi	Citrus × aurantium L.	Fructus aurantii immaturus	Citrus	Rutaceae	9	Fruit
Shaoyao	Paeonia × suffruticosa Andrews	Paeonia lactiflora	Paeonia	Paeoniaceae	9	Root
Ban xia	Pinelliaternate (Thunb.) Makino	Rhizomapinelliae	pinellia	Araceae	9	Root and rhizoma
Da huang	Rheum palmatum L.	Rheum palmatum	Rheum	polygonaceae	6	Root and rhizome
Sheng jiang	Zingiber officinale Roscoe	Rhizoma zingiberis recens	Zingiber Adans .	zingiberaceae	15	Root and rhizome
Da zao	Ziziphus jujube MIll	Fructus jujuba	Ziziphus	Rhamnales	20	fruit

Table 2 Primer sequences used for real time-polymerase chain reaction

Gene	Forward primer	Reverse primer	Cycles	Temperature, °C
IL-6	5’-GAAGTAGGGAAGGCCGTGG-3’	5’-CTCTGCAAGAGACTTCCATCCAGT-3’	40	60
MCP-1	5’-GTTGGCCTCAGCCAGATGCA-3’	5’-AGCGTACTCATTGGGATCATCTTG-3’	40	60
MIP-1α	5’-TGCCCTTGCTHTTCTTCTCTGCACCATGGC-3’	5’-GGCAAATTCCACGAAAATTCATTGCTGACT-3’	40	60
β-actin	5’-CATCCTGCGTCTGGACCT-3’	5’-TCAGGAGGAGCAATGATCTTG-3’	40	60