Circular RNA hsa_circ_0000745 may serve as a diagnostic marker for gastric cancer

Table 1 The three differently expressed circRNAs in human gastric cancer and colorectal cancer samples

CircRNA	Chrom	TxStart	TxEnd	circBaseID	Best_transcript	Gene name	Catalog
Chr16: 30740287-30740893|+	16	30740286	30740893	266	NM_006662	SRCAP	Exonic
Hsa_circ_0000745	17	20107645	20109225	790	NM_152904	SPECC1	Exonic
Hsa_circ_0085616	8	131370262	131374017	127	NM_001247996	ASAP1	Exonic

Chrom: Chromosome; Tx: Transcription.

Table 2 Primers used for quantitative reverse transcription-polymerase chain reaction analysis of circular RNA and mRNA levels

Target ID	Primer sequence, 5’-3’	Product size in bp
Circ_0000745	F: GTTGAAAGTAGCCCGAGCAG	204
	R: ACGTGGCACAGACCTCTCTC
Circ_0085616	F: GACTACAACTCGCCCACCAC	200
	R: TCCATTTCTGGGCCATAATC
Chr16:30740286-30740893|+	F: CCTTTGCACCGTATTGTGTG	199
	R: GGCAGGGTACAGAAATCCAG
GAPDH	F: CTCGCTTCGGCAGCACA	122
	R: AACGCTTCACGAATTTGCGT

bp: Base pairs; F: Forward; R: Reverse.

Table 3 Relationship of hsa_circ_0000190 expression levels (2^-ΔΔCt) in gastric cancer tissues with clinicopathological factors of patients with gastric cancer

Clinicopathological factor		Tissue hsa_circ_0000745		Plasma hsa_circ_0000745
	n	Mean ± SD	P value	mean ± SD	P value
Age (yr)			0.757		0.195
< 60	33	0.77 ± 0.83		0.90 ± 0.96
≥ 60	27	0.58 ± 0.60		0.69 ± 0.53
Sex			0.203		0.252
Male	43	0.59 ± 0.67		0.68 ± 0.60
Female	17	0.92 ± 0.85		1.13 ± 1.12
Diameter in cm			0.168		0.127
≥ 5	26	0.76 ± 0.77		0.87 ± 0.76
< 5	34	0.63 ± 0.71		0.76 ± 0.84
Differentiation			0.012a		0.087
Well	2	0.96 ± 1.10		0.89 ± 0.44
Moderate	25	0.65 ± 0.71		0.70 ± 0.66
Poor	33	0.70 ± 0.77		0.88 ± 0.92
Lymphatic metastasis			0.064		0.087
Present	44	0.71 ± 0.71		0.85 ± 0.78
Absent	16	0.61 ± 0.81		0.69 ± 0.88
TNM stage			0.056		0.046a
I-II	14	0.63 ± 0.81		0.60 ± 0.53
III-IV	46	0.70 ± 0.72		0.87 ± 0.87
CEA in ng/mL			0.077		0.130
≥ 5	10	0.79 ± 0.72		0.65 ± 0.59
< 5	50	0.66 ± 0.74		0.85 ± 0.78

^aindicated statistical significance. CEA: Carcinoembryogenic antigen.