Notch signaling mediated by TGF-β/Smad pathway in concanavalin A-induced liver fibrosis in rats

Table 1 Sequences of primers for quantitative real-time reverse transcription polymerase chain reaction

Gene	Forward	Reverse
Notch1	5’-TCGTGCTCCTGTTCTTTGTG-3’	5’-TTCTCTCCGCTTCTTCTTGC-3’
Notch2	5’-TGATGAGCAGGAACAGGAGA-3’	5’-ATGAGAAGCCAGGAGAGCAG-3’
Notch3	5’-ATACCCACTACGGGATGTGC-3’	5’-AGGACGAAGATGACCAGCAG-3’
Notch4	5’-GGATGAATGTCGGAGTGACC-3’	5’-GGCTACACAAGGGAACCTCA-3’
Jagged1	5’-CAGTGGCTTGGGTCTGTTG-3’	5’-CATTGTTGGTGGTGTTGTCC-3’
Hes1	5’-GTGGGTCCTAACGCAGTGTC-3’	5’-GTCAGAAGAGAGAGGTGGGCTA-3’
Hes5	5’-ATGCTCAGTCCCAAGGAGAA-3’	5’-CTCCAGCAGCAGTTTCAGC-3’
TGF-β1	5’-ATTCCTGGCGTTACCTTGG-3’	5’-AGCCCTGTATTCCGTTCTCT-3’
Smad2	5’-CGGCTGAACTGTCTCCTACC-3’	5’-AGGTCTCTCCAACCCTCTGG-3’
Smad3	5’-GGTAAAGGATTGCCACCAAA-3’	5’-GAACAGCCAGGAAAGGGACT-3’
β-actin	5’-CACCCGCGAGTACAACCTTC-3’	5’-CCCATACCCACCATCACACC-3’

Table 2 Levels of serum biochemical markers in rats

Group	ALT (U/L)	AST (U/L)	Albumin (g/L)
Control	47.32 ± 5.21	167.68 ± 14.31	34.23 ± 2.62
Model	75.13 ± 9.68a	252.06 ± 22.83a	25.60 ± 1.90a

Data are presented as mean ± SD.

^aP < 0.05 vs control, n = 12 per group. ALT: Alanine aminotransferase; AST: Aspartate aminotransferase.