Basic Study
Copyright ©The Author(s) 2016.
World J Gastroenterol. Dec 28, 2016; 22(48): 10545-10556
Published online Dec 28, 2016. doi: 10.3748/wjg.v22.i48.10545
Table 1 Morphological characteristics, hemodynamic and biochemical parameters of the Sham, partial portal vein ligation, and bile duct ligation groups
ShamPPVLBDL
Body weight gain (g)39 ± 635 ± 610 ± 8ac
Spleen weight (g)0.7± 0.11.3 ± 0.1a1.4 ± 0.2a
Liver weight (g)11.6 ± 0.610.9 ± 0.716.9 ± 0.7ac
Mean arterial pressure (mmHg)116 ± 995 ± 5a92 ± 6a
Portal pressure (mmHg)7 ± 117 ± 2a19 ± 3a
Bilirubin (mg/dL)0.12 ± 0.030.15 ± 0.039.91 ± 0.07ac
Creatinine (mg/dL)0.75 ± 0.070.73 ± 0.060.78 ± 0.06
Table 2 pD2 values and maximal responses of the concentration-response curves to KCl (10-120 mmol/L) in renal arteries from Sham, partial portal vein ligation and bile duct ligation groups
npD2Emax (mg)
Sham81.49 ± 0.011018 ± 83
PPVL81.46 ± 0.011050 ± 131
BDL81.46 ± 0.01762 ± 59ac
Table 3 pD2 values and maximal responses of the concentration-response curves to NG-nitro-L-arginine methyl ester and asymmetric dimethylarginine in renal arteries from Sham, partial portal vein ligation, and bile duct ligation groups, after precontraction with norepinephrine
npD2Emax (mg)
Sham
L-NAME85.35 ± 0.17370 ± 36
ADMA84.20 ± 0.08e400 ± 26
PPVL
L-NAME85.30 ± 0.16365 ± 30
ADMA84.11 ± 0.09e388 ± 25
BDL
L-NAME85.24 ± 0.16285 ± 29ac
ADMA84.79 ± 0.16ace310 ± 27ac
Table 4 pD2 and maximal response values for the concentration-response curves to acetylcholine in renal arteries from Sham, partial portal vein ligation, and bile duct ligation groups in the absence (Control) and in the presence of NG-nitro-L-arginine methyl ester or asymmetric dimethylarginine
AcetylcholinenpD2Emax (%)
Sham
Control87.95 ± 0.0893 ± 3
L-NAME (3 × 10-4 mol/L)87.13 ± 0.28a24 ± 7a
ADMA (3 × 10-4 mol/L)87.27 ± 0.13ac60 ± 3ac
PPVL
Control87.92 ± 0.0894 ± 2
L-NAME (3 × 10-4 mol/L)87.24 ± 0.11a21 ± 3a
ADMA (3 × 10-4 mol/L)87.18 ± 0.15a65 ± 6ac
BDL
Control87.91 ± 0.1095 ± 3
L-NAME (3 × 10-4 mol/L)87.13 ± 0.35a24 ± 8a
ADMA (3 × 10-4 mol/L)87.17 ± 0.22a32 ± 6ace