Mutations in pre-core and basic core promoter regions of hepatitis B virus in chronic hepatitis B patients

Table 1 Hepatitis B virus DNA content and liver function indexes

Parameter	HBV DNA (IU/mL)	ALT	AST	TBIL	DBIL	ALP	GGT	ALB	TP
Median	6.64	45	55	15.8	5.05	96.0	36	43	72
Interquartile range	2.48	69	57	9.8	4.35	51.3	52	5.3	7

Normal distribution test, P < 0.10 for all. HBV: Hepatitis B virus.

Table 2 Distribution of mutations in the basic core promoter and pre-C regions of hepatitis B virus according to hepatitis B e antigen status

		BCP region		Total	χ2(corrected)	P value	Pre-C region		Total	χ2(corrected)	P value
		Mutations	WT				Mutations	WT
HBeAg	+	46	24	70	2.43	0.12	24	46	70	26.62	0.00
	-	22	4	26			25	1	26
Total		68	28	96			49	47	96

BCP: Basic core promoter; WT: Wild-type; HBeAg: Hepatitis B e antigen.

Table 3 Comparison of hepatitis B virus DNA contents between patients with mutant basic core promoter or pre-C loci and those with wild-type loci

	BCP region	No. of cases	Mean rank	Wilcoxon	P value	Pre-C region	No. of cases	Mean rank	Wilcoxon	P value
DNA content	Mutations	68	42.74			Mutations	49	36.79
	WT	28	62.48	2906.5	0.00	WT	47	60.71	1802.5	0.00
	Total	96				Total	96

BCP: Basic core promoter; WT: Wild-type.

Table 4 Comparison of liver function between patients with mutant or wild-type basic core promoter loci

Item	AST				GGT
BCP region	No. of cases	Mean rank	Wilcoxon	P value	Mean rank	Wilcoxon	P value
WT	28	33.75			33.0
Mutant	68	53.96	945.0	0.00	54.8	924.0	0.00
Total	96

BCP: Basic core promoter; WT: Wild-type.

Table 5 Comparison of liver function between patients with mutant or wild-type pre-C loci

Item	AST				GGT
	No. of cases	Mean rank	Wilcoxon	P value	Mean rank	Wilcoxon	P value
pre-C loci
WT	47	40.55			41.33
Mutant	49	55.29	1906.0	0.00	55.38	1942.5	0.01
Total	96

WT: Wild-type.

Table 6 Consistency of two methods for detection of mutations in the basic core promoter and pre-C regions of hepatitis B virus

		BIOTGENE for BCP region		Total	Kappa	P value			BIOTGENE for pre-C region		Total	Kappa	P value
DAAN for BCP region		WT	Mutant				DAAN for		WT	Mutant
	WT	23	2	25			pre-C region	WT	29	5	34
	Mutant	1	50	51	0.91	0.00		Mutant	11	31	42	0.58	0.00
	Total	24	52	76				Total	40	36	76

BCP: Basic core promoter; WT: Wild-type.