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Copyright ©The Author(s) 2015.
World J Gastroenterol. Nov 14, 2015; 21(42): 11954-11963
Published online Nov 14, 2015. doi: 10.3748/wjg.v21.i42.11954
Table 1 Characteristics of isothermal amplification methods and polymerase chain reaction reviewed in this article
MethodTemperature requirement (°C)No. of enzymesPrimer designProduct detection methodRapid detection possibilityTolerance to biological components
PCR55-951SimpleGE, ELISA, Real-timeYesNo
LAMP60-651ComplexGE, turbidity, Real-timeYesYes
TMA50-602SimpleGE, ELISA, Real-time, ECLYesNo
NASBA37-412 or 3SimpleGE, ELISA, Real-time, ECLYesNo
RCA371SimpleGE, Real-timeYesNo
Table 2 Quantitative detection methods for hepatitis B virus discussed in this review
MethodTargetDetection rangeRef.
ArchitectHBsAg0.05-250.0 IU/mL[12]
UV spectrophotometryHBV DNA0.4-15000 ng/µL[23]
In-house assays based on real-time PCRHBV DNA101-108 copies/reaction[27]
HBV DNA50-108 IU/mL[19]
Digital PCRHBV DNASingle copy[39]
LAMPHBV DNA48-108 IU/mL[43]
TMAHBV DNA5 × 103-5 × 108 GE/mL[47]
NASBAHBV DNA103-109 copies/mL[52]
HBV DNA102-109 IU/mL[53]
RCAHBV DNA103-108 IU/mL[57]
Electrochemical biosensorsHBV DNA3.96 × 10-7-1.32 × 10-6 mol/L[65]
HBV DNA1.01 × 10-8-1.62 × 10-6 mol/L[66]
QCM biosensorsHBV DNA0.02-0.14 mg/mL[67]
HBV DNA8.6 pg/L1[68]
Microcantilever biosensorsHBV DNA23.1 fmol/L-2.31 nmol/L[69]
SPR biosensorsHBV DNA2 fg/mL1[45]