Transplantation of insulin-producing cells to treat diabetic rats after 90% pancreatectomy

Table 1 Sequences of reverse transcription-polymerase chain reaction and real-time reverse transcription-polymerase chain reaction primers

Gene	Sequence	Fragment length (bp)	Annealing temperature (°C)
Insulin	F: 5’- ATC AAG CAG ATC ACT GTC CTT CT-3’	162	60
	R: 5’- GAG AGC TTC CAC CAG GTG TG-3’
PDX1	F: 5’- TCC CAT GGA TGA AGT CTA CC-3’	246	60
	R: 5’- TGT CCT CCT CCT TTT TCC AC-3’
Ngn3	F: 5’- CGC CGG TAG AAA GGA TGA C-3’	316	60
	R: 5’- GAG TTG AGG TTG TGC ATT CG-3’
Glut2	F: 5’- AGT ACA ATG ACA GAA GAT AAG GTC-3’	423	60
	R: 5’- AGC TCC AAC TAA TGA CAG AAT G-3’
Pax4	F: 5’- ATC CTT AAG GTA TCT AAT GGC TG-3’	461	60
	R: 5’- GCC ACT GAA TCA GGA TAC TGC-3’
GAPDH	F: 5’-AGA AGG CTG GGG CTC ATT TG-3’	258	52
	R: 5’-AGG GCC ATC CAC AGT CTT C-3’

F: Forward; R: Reverse.