Alternative splicing of VEGFA, APP and NUMB genes in colorectal cancer

Table 1 Patient characteristics

Characteristics	n (%)
Sex
Male	21 (52.5)
Female	19 (47.5)
Primary tumor location
Ascending colon	5 (12.5)
Transverse colon	7 (17.5)
Sigmoid colon	15 (37.5)
Rectum	13 (32.5)
TNM stage
I	7 (17.5)
II	14 (35.0)
III	13 (32.5)
IV	6 (15.0)
T factor
T1	3 (7.5)
T2	7 (17.5)
T3	11 (27.5)
T4	19 (47.5)
Lymphatic invasion
N0	19 (47.5)
N1	9 (22.5)
N2	12 (30.0)
Distant metastasis
M0	34 (85.0)
M1	6 (15.0)

TNM: Tumor-node-metastasis.

Table 2 Primers for quantitative reverse transcription-polymerase chain reaction

Gene	Primers	Sequence (5'-3')	Products (bp)
GAPDH	Forward	TGAAGGTCGGAGTCAACGGA	225
	Reverse	CCTGGAAGATGGTGATGGGAT
VEGFA	Forward	TTGCTGCTCTACCTCCACCAT	270
	Reverse	GGTGATGTTGGACTCCTCAGTG
APP	Forward	GGCGGAGCAGACACAGACTA	131
	Reverse	ACCTCATCACCATCCTCATCGT
NUMB	Forward	GCTGGATCTGTCACTGCTTCAT	118
	Reverse	CCACATTCCTTCTCCCGCTTC
VEGFA165b	Forward	TCAGAGCGGAGAAAGCATTTGT	130
	Reverse	TCCTGGTGAGAGATCTGCAAGT

Table 3 Primers for polymerase chain reaction-restriction fragment length polymorphism

Gene	Primers	Sequence (5'-3')	Products (bp)
GAPDH	Forward	FAM-TGAAGGTCGGAGTCAACGGA	225
	Reverse	CCTGGAAGATGGTGATGGGAT
VEGFA	Forward	FAM-TGAGCTTCCTACAGCACAAC	206/338/410
	Reverse	TCGATGGTGATGGTGTGGTG
APP	Forward	FAM-CCTACGAAGAAGCCACAGAG	162/330/387
	Reverse	GGGCATGTTCATTCTCATCC
NUMB	Forward	FAM-TGCTCCGATGACCAAACCAG	157/301
	Reverse	CACCTCTTCTAACCATCGGTC