Prognostic value of neutrophil distribution in cholangiocarcinoma

Table 1 Relationship between CD15 expression and clinicopathological characteristics in tumor tissues n (%)

Clinicopathological parameter	Group	n	CD15 expression in tumor tissues
			High	Low	χ2	P value
Age (yr)	< 60	119	92 (77.3)	27 (22.7)	1.569	0.210
	≥ 60	135	95 (70.4)	40 (29.6)
Gender	Male	170	128 (75.3)	42 (24.7)	0.740	0.390
	Female	84	59 (70.2)	25 (29.8)
Differentiation	Well differentiated	17	11 (64.7)	6 (35.3)	1.093	0.579
	Moderately differentiated	140	102 (72.9)	38 (27.1)
	Poorly differentiated	97	74 (76.3)	23 (23.7)
TNM stage	I	136	92 (67.6)	44 (32.4)	8.589	0.032
	II	71	54 (76.1)	17 (23.9)
	III	34	31 (91.2)	3 (8.8)
	IV	13	10 (76.9)	3 (23.1)
Lymphatic metastasis	Metastasis	39	33 (84.6)	6 (15.4)	2.867	0.090
	No metastasis	215	154 (71.6)	61 (28.4)
Neural invasion	Yes	82	56 (68.3)	26 (31.7)	1.771	0.183
	No	172	131 (76.2)	41 (23.8)
Vascular invasion	Yes	14	10 (71.4)	4 (28.6)	0.037	0.765
	No	240	177 (73.8)	63 (26.2)
Infiltration	Non- infiltrative	148	106 (71.6)	42 (28.4)	0.731	0.393
	Infiltrative	106	81 (76.4)	25 (23.6)
Size	< 3 cm	138	95 (68.8)	43 (31.2)	3.557	0.059
	≥ 3 cm	116	92 (79.3)	24 (20.7)
Edge	Positive	63	46 (73.0)	17 (27.0)	0.016	0.900
	Negative	191	141 (73.8)	50 (26.2)
Position	Intrahepatic	69	52 (75.4)	17 (24.6)	0.148	0.701
	Extrahepatic	185	135 (73.0)	50 (27.0)
Surgical approach	Radical	243	179 (73.7)	64 (26.3)	0.000	1.000
	Palliative	11	8 (72.7)	3 (27.3)

TNM stage: Tumor node metastasis stage.

Table 2 CD15 expression in tumor tissues and adjacent tissues

Group	n1	High CD15 expression	Low CD15 expression
Tumor tissues	254	187	67
Adjacent tissues	238	130	108

¹The number of cases is different from the previous because of off-chip effects during the dyeing process or no tumor tissue.

Table 3 Multivariate survival analysis of CD15 expression in cancer tissues and clinicopathological features

Parameters	Risk of recurrence				Risk of death
	P value	Exp(B)	95%CI for Exp(B)		P value	Exp(B)	95%CI for Exp(B)
			Lower	Upper			Lower	Upper
Differentiation	0.004	1.545	1.148	2.079	0.007	1.43	1.103	1.853
TNM stage	0.001	1.535	1.185	1.989	0.013	1.324	1.061	1.652
Lymphatic metastasis	0.854	0.952	0.562	1.611	0.114	1.427	0.918	2.217
Neural invasion	0.532	1.134	0.764	1.684	0.827	1.039	0.736	1.468
Vascular invasion	0.227	0.594	0.255	1.382	0.965	0.986	0.523	1.859
Infiltration	0.742	0.928	0.595	1.448	0.585	1.112	0.759	1.63
Size	0.943	1.014	0.692	1.487	0.758	0.949	0.679	1.326
Edge	0.014	1.014	0.692	1.487	0.007	1.641	1.148	2.347
Position	0.551	0.859	0.521	1.416	0.466	0.849	0.546	1.319
Surgical approach	0.575	1.258	0.564	2.804	0.161	1.62	0.826	3.178
CD15	0.073	1.462	0.965	2.215	0.012	1.601	1.107	2.314

TNM: Tumor node metastasis.