Basic Study
Copyright ©The Author(s) 2015.
World J Gastroenterol. Apr 28, 2015; 21(16): 4829-4839
Published online Apr 28, 2015. doi: 10.3748/wjg.v21.i16.4829
Table 1 Initial and final weight; length, width and area of the jejunum and thickness of the external muscle layer of the jejunum in rats from the control group and infected group
ParametersCGIG
Initial body weight (g)254.8 ± 15.90262.4 ± 11.29
Final body weight (g)394.1 ± 14.941419.8 ± 31.531
Length (cm)108.4 ± 5.07110.4 ± 6.83
Width (cm)0.96 ± 0.150.96 ± 0.15
Area (cm2)104.35 ± 19.15106.82 ± 23.53
Muscular fold thickness (μm)51.40 ± 12.34177.02 ± 42.711
Table 2 Population density and morphometric analysis of the cell body of myenteric neurons labeled with Giemsa, NADHd-p and NOS immunohistochemistry in healthy and infected rats with the ME-49 strain of Toxoplasma gondii during 36 d
MeasuresGIEMSA
NADHd-p
NOS
CGIGCGIGCGIG
Number of neurons/cm239839.3 ± 5362.3126766.6 ± 2177.6111484.5 ± 2211.813155.9 ± 1319.37959.0 ± 1290.4110893 ± 1156.31
Projection of number of neurons for the organ4157229.4 ± 559555.112859215.3 ± 232611.311198410.1 ± 230807.11405318.8 ± 157570.2735729.9 ± 125754.71939630.1 ± 156871.21
Cell body (μm2)232.5 (187.2-286.0)1248.2 (204.4-293.0)1146.7 (96.8-202.0)1155.8 (108.0-14.8)1328.1 ± 2.431344.1 ± 2.21
Nucleus (μm2)99.9 (82.5-115.3)1102.6 (88.5-119.2)164.9 (43.7-88.5)176.9 (54.1-99.2)197.5 ± 0.701100.9 ± 0.671
Cytoplasm (μm2)130.8 (99.4-175.1)1141.6 (112.1-182.4)179.2 (48.0-115.9)174.2 (48.2-117.4)1230.6 ± 2.11243.8 ± 1.91
Ratio0.42 (0.37-0.48)0.41 (0.37-0.47)0.44 (0.37-0.51)10.48 (0.40-0.57)10.30 ± 0.0020.31 ± 0.003
Table 3 Smaller, medium and larger areas (μm²) of VIPergic varicosities and number of enteric glial cells/mm² from the jejunal myenteric plexus of rats from the Control Group and from the Group infected with oocysts of Toxoplasma gondii ME-49 strain
ParametersCGIG
Smaller areas (μm2)0.46 ± 0.1010.80 ± 0.161
Larger areas (μm2)21.68 ± 5.1324.50 ± 0.90
Medium areas (μm2)9.28 ± 5.849.51 ± 5.99
Enteric glial cells/mm²1011.18 ± 25.481807.24 ± 39.981