Basic Study
Copyright ©The Author(s) 2015.
World J Gastroenterol. Mar 28, 2015; 21(12): 3509-3518
Published online Mar 28, 2015. doi: 10.3748/wjg.v21.i12.3509
Table 1 Selected primary antibodies in the study
AntigenImmunizing antigenHost speciesDilution
Source/Catalog No.
COMTSynthetic peptide corresponding to amino acid residues 60-76 of rat COMT conjugated to KLHRabbit1:2001:1000Santa Cruz/sc-25844
COMTSynthesized peptide derived from human COMTRabbit1:4001:1000Sigma/SAB4500401
GAPDHA synthetic peptide containing 314-333 amino acids of mouse GAPDH with a 407972 gene IDRabbit-1:5000Sigma/G9545
NFPellet of pig brain cold stable proteins after depolymerization of microtubulesMouse1:400-Abcam/ab7794
NFRecombinant C-terminal segment of rat NF-MRabbit1:400-Novus/NB300-133
Table 2 Selected secondary antibodies in the study
AntibodyConjugationDilutionSource/Catalog No.
Goat anti-rabbit IgGAlexa Fluor 4881:100Beyotime/A0423
Goat anti-rabbit IgGAlexa Fluor 4881:100Beyotime/A0428
Donkey anti-rabbit IgGIRDyeTM 8001:10000Rockland/16747