Prevalence of human papillomavirus in esophageal carcinoma in Tangshan, China

doi:10.3748/wjg.v21.i10.2905

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Mar 14, 2015; 21(10): 2905-2911
Published online Mar 14, 2015. doi: 10.3748/wjg.v21.i10.2905

Table 1 Primers used in PCR amplification of human papillomavirus DNA

Primer	Sequence, 5’-3’	Annealing temperature,°C	PCR product, bp
PC04	CAACTTCATCCACGTTCACC	62	150
GH20	GAAGAGCCAAGGACAGGTAC	62	150
GP5+	TTTGTTACTGTGGTAGATACTAC	55	150
GP6+	CTTATACTAAATGTCAAATAAAAAG	55	150
HPV16E6F	CAACAAGACATACATCGACC	60	350
R	CAACAAGACATACATCGACC	60	350
HPV18E6F	CACTTCACTGCAAGACATAGA	55	350
R	GTTGTGAAATCGTCGTTTTTCA	55	350

Table 2 Polymerase chain reaction detection frequency of human papillomavirus using different primers

Primer set	Tested cases, n	Positive cases, n	Positive cases
PC04/GH20	198	196	> 98.00%
GP5+/GP6+	198	158	79.79%
HPV 16	198	80	40.40%
HPV 18	198	94	47.47%
GP+ and HPV16/18-¹	198	13	6.50%
HPV 16 and 18	198	55	27.77%
HPV 16 or 18	198	119	60.10%
GP and HPV 16 and 18	198	48	24.24%
GP+/HPV16+/HPV18+²	198	185	93.00%

¹GP5+/GP6+ positive but human papillomavirus (HPV) 16/18 negative;

²GP5+/GP6+ positive or HPV 16 positive or HPV 18 positive.

Citation: Mehryar MM, Li SY, Liu HW, Li F, Zhang F, Zhou YB, Zeng Y, Li JT. Prevalence of human papillomavirus in esophageal carcinoma in Tangshan, China. World J Gastroenterol 2015; 21(10): 2905-2911
URL: https://www.wjgnet.com/1007-9327/full/v21/i10/2905.htm
DOI: https://dx.doi.org/10.3748/wjg.v21.i10.2905