T3-induced liver AMP-activated protein kinase signaling: Redox dependency and upregulation of downstream targets

Table 1 Primers for SYBR green based qRT-PCR assays

mRNA	Forward primer	Reverse primer
Rat ACCα	GAC GTT CGC CAT AAC CAA GT	CTG CAG GTT CTC AAT GCA AA
Rat ACCβ	CCT GTA GAT GCC AGT CAG CA	AGT TCT GGG GAG GAA CAG GT
Rat ACOT2	TCA GGA TGA CCA CAACTG GA	ATG TTA GAC CCC ACC AGG AG
Rat ACOX1	CTG ATG AAA TAC GCC CAG GT	GGT CCC ATA CGT CAG CTT GT
Rat AMPK	TTT GCC TAG AAT CCC CAC AG	TAA GGA GCC CAG AAA ACA GC
Rat β-actin	AGC CAT GTA CGT AGC CAT CC	CTC TCA GCT GTG GTG GTG AA
Rat CaMKKβ	GGA TTG TGG TGC CTG AAA TC	AGG CTG GGA ATG TGT TTG AC
Rat CPT-1α	CAG CTC GCA CAT TAC AAG GA	TGC ACA AAG TTG CAG GAC TC
Rat CREB	TCA GCC GGG TAC TAC CAT TC	CCT CTC TCT TTC GTG CTG CT
Rat PGC-1α	TCC ACA GAT TCA AGC CAG TG	TGA CCG AAG TGC TTG TTC AG
Rat RPS23	GTA GGG GTT GAA GCC AAA CA	CAC CTT AAA GCG GAC TCC AG
Rat TAK1	AAC AAG TCC CTG CCA CAA AC	CAT CCT CTT GCC CTC AGA AG

Sequences are listed in the 5’→ 3’ direction. ACCα(β): Acetyl-CoA carboxylase α and β; ACOT2: Acyl-CoA thioesterase; ACOX1: acyl-CoA oxidase 1; AMPK: AMP-activated protein kinase; CaMKKβ: Ca²⁺-Calmodulin-dependent protein kinase kinase-β; CPT-1α: Carnitine palmitoyltransferase-1α; CREB: cAMP-response element-binding protein; PGC-1α: Peroxisome proliferator-activated receptor-γ coactivator-1α; RPS23: Ribosomal protein S23; TAK1: Transforming growth factor-β-activated kinase-1.