Molecular detection of monocyte chemotactic protein-1 polymorphism in spontaneous bacterial peritonitis patients

Table 1 Baseline demographic and clinical characteristics of the studied groups

	Control group(n = 20)	Group I (SBP)(n = 25)	Group II (cirrhotic)(n = 25)
Age (yr)	48.28 ± 4.56	51.24 ± 9.3	47.08 ± 12.9
Sex (male)	15 (75)	18 (72)	15 (60)
BMI (kg/m2)	28.42 ± 2.33	27.94 ± 2.1	28.2 ± 1.8
DM (Yes)	0	5 (20)	8 (32)
GIT bleeding (Yes)	0	5 (20)	5 (20)
Hepatic encephalopathy (Yes)	0	9 (36)	7 (28)
Duration of liver cirrhosis (yr)	0	4.46 ± 5	4.04 ± 3.26
Duration of ascites (yr)	0	1 ± 2.01	1.71 ± 1.54
Hemoglobin (g/dL)	12.6 ± 1.6	10.27 ± 1.95b	9.5 ± 2.22b
Platelets (103/μL)	158.4 ± 12.8	146.6 ± 90.2	118.6 ± 35.9b
TLC (103/μL)	6.3 ± 0.97	5.82 ± 3.05	6.7 ± 2.58
Serum albumin (g/dL)	4.34 ± 0.62	2.26 ± 0.39b	2.3 ± 0.46b
Total bilirubin (mg/dL)	1.036 ± 0.064	5.42 ± 8.7b	2.69 ± 2.65b
Direct bilirubin (mg/dL)	0.176 ± 0.078	3.07 ± 5.3b	1.44 ± 1.58b
Urea (mg/dL)	17.4 ± 3.3	59.45 ± 26.6ab	42.8 ± 24.49b
Creatinine (mg/dL)	0.86 ± 0.208	1.96 ± 1.79b	1.5 ± 0.95b
AST (IU/L)	47.96 ± 7.7	69.86 ± 38.03a	85.8 ± 52.99b
ALT (IU/L)	23.79 ± 7.5	39.7 ± 16.03b	40.17 ± 24.76a
ALP (IU/L)	95.5 ± 19.8	167.9 ± 69.49b	101.4 ± 39.67
INR	0.996 ± 0.13	1.84 ± 0.59b	1.66 ± 0.40b
MCP-1 gene expression in whole blood	0.131 ± 0.0367	1.04 ± 0.119bd	0.112 ± 0.046
Serum IL-10 (pg/mL)	14.48 ± 3.29	29.26 ± 7.037bd	15.91 ± 4.53
PMN count in ascites (cells/mm3)	-	1194.6 ± 1187.6d	110.3 ± 60.89
Serum-Ascites Albumin gradient (SAAG) (g/dL)	-	1.34 ± 0.107d	1.67 ± 0.32
Ascitic IL-10 (pg/mL)	-	60.07 ± 12.67d	16.86 ± 5.2
Ascitic MCP-1 gene expression	-	2.251 ± 1.039d	1.5 ± 0.59

^aP < 0.05,

^bP <0.01 vs control group;

^dP <0.01, group I vs group II. Results are expressed as mean ± SD or frequency (%) as required. BMI: Body mass index; DM: Diabetes mellitus; GIT: Gastrointestinal; TLC: Total lymphocyte count; AST: Aspartate aminotransferase; ALT: Alanine aminotransferase; ALP: Alkaline phosphatase; INR: International normalized ratio; MCP-1: Monocyte chemotactic protein-1; SBP: Spontaneous bacterial peritonitis.

Table 2 MCP-1 genotypes in the studied groups

	Control group(n = 20)	Group I (SBP)(n = 25)	Group II (cirrhotic)(n = 25)
Genotype
AA	11 (55)	2 (8.0)b	4 (16)b
AG	8 (40)	19 (76.0)b	5 (20)bd
GG	1 (5)	4 (16.0)	16 (64)bd
Allele
A	30 (75.0)	23 (46.0)bd	13 (26.0)b
G	10 (25.0)	27 (54.0)b	37 (74.0)bc

^aP < 0.05,

^bP <0.01 vs control group;

^cP < 0.05,

^dP <0.01, group I vs group II. Results are expressed as frequency (%). MCP-1: Monocyte chemotactic protein-1.