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Molecular detection of monocyte chemotactic protein-1 polymorphism in spontaneous bacterial peritonitis patients
Maysa Kamal Salama, Dina Sabry, Mohamed AS Al-Ghussein, Rasha Ahmed, Sayed AbdAllah, Fatma Mohamed Taha, Wael Fathy, Miriam Safwat Wadie, Mona Nabih, Amr Abul-Fotouh, Tarneem Darwish
Maysa Kamal Salama, Dina Sabry, Fatma Mohamed Taha, Miriam Safwat Wadie, Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Cairo University, Cairo11562, Egypt
Mohamed AS Al-Ghussein, Biochemistry Department, Faculty of Pharmacy, Al-Azhar University, Gaza 1277, Palestine
Rasha Ahmed, Amr Abul-Fotouh, Tropical Medicine Department, Faculty of Medicine, Cairo University, Cairo11562, Egypt
Sayed AbdAllah, Mona Nabih, Internal Medicine Department, Faculty of Medicine, Cairo University, Cairo 11562, Egypt
Wael Fathy, Tropical Medicine Department, Faculty of Medicine, Bny Swif University, Bny Swif 62511, Egypt
Tarneem Darwish, Biomedical Informatics and Biostatistics Department, Faculty of Medicine, Cairo University, Cairo 11562, Egypt
Author contributions: Salama MK participated in the design of the study, drafted the article and revised it critically for important intellectual content; Sabry D carried out PCR and RNA extraction and real-time PCR and participated in manuscript writing and submissions; Al-Ghussein MAS participated in biotechnology procedures and carried out manuscript writing and submissions as a corresponding author; Ahmed R participated in the design of the study, revised and edited the manuscript critically for important intellectual content; AbdAllah S carried out the collection and monitoring of patient and control samples; Taha FM carried out the MCP-1 and IL-10 detection and manuscript writing; Fathy W provided the study materials, technical and logistic support and carried out hospital procedures; Wadie MS carried out the sample preparation, detection and provided vital reagents; Nabih M participated in clinical assessment of the patients; Abul-Fotouh A carried out manuscript revision; Darwish T carried out the statistical calculations; and all authors read and approved the final manuscript.
Correspondence to: Mohamed AS Al-Ghussein, PhD, Lecturer of Biochemistry and Medical Biochemistry, Biochemistry Department, Faculty of Pharmacy, Al-Azhar University, Talatini st., Gaza strip, Gaza 1277, Palestine. mohamedghussein@yahoo.com
Telephone: +972-2-01202717604 Fax: +972-2-0223658095
Received: October 28, 2013
Revised: May 5, 2014
Accepted: May 26, 2014
Published online: September 7, 2014