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©2014 Baishideng Publishing Group Inc.
World J Gastroenterol. Sep 7, 2014; 20(33): 11762-11769
Published online Sep 7, 2014. doi: 10.3748/wjg.v20.i33.11762
Published online Sep 7, 2014. doi: 10.3748/wjg.v20.i33.11762
Table 1 Inter-assay analysis of hepatitis B virus DNA levels of reference sera measured by the Abbott and Da-an assays
| Expected concentration of HBV DNA standard (log IU/mL) | Abbott assay (log IU/mL)(mean ± SD) | CV | Da-an assay (log IU/mL) (mean ± SD) | CV |
| L0: 8.34 (7.89-8.79) | 8.64 ± 0.07 | 0.8% | 8.13 ± 0.17 | 2.0% |
| L1: 7.62 (7.17-8.07) | 7.91 ± 0.04 | 0.5% | 7.65 ± 0.10 | 1.3% |
| L2: 6.65 (6.20-7.10) | 6.89 ± 0.05 | 0.7% | 6.79 ± 0.01 | 0.2% |
| L3: 5.67 (5.22-6.12) | 5.89 ± 0.01 | 0.2% | 6.53 ± 0.65 | 10.0% |
| L4: 4.72 (4.26-5.17) | 4.92 ± 0.08 | 1.7% | 5.27 ± 0.21 | 3.9% |
| L5: 3.64 (3.18-4.09) | 3.85 ± 0.24 | 1.1% | 3.96 ± 0.04 | 1.0% |
| L6: 2.04 (1.59-2.49) | 2.33 ± 0.07 | 3.0% | 2.19 ± 0.01 | 0.4% |
Table 2 Comparison between hepatitis B virus DNA levels of 180 serum samples from 48 patients with chronic hepatitis B measured with the Abbott and Da-an real-time polymerase chain reaction assays
| Abbott RealTime assay result(log IU/mL) | Da-an real-time HBV DNA assay result (log IU/mL) | |||
| ≥8.00 | ≥3.00-< 8.00 | < 3.00 (negative) | Total | |
| > 9.00 | 0 | 3 | 0 | 3 |
| ≥ 1.18- ≤ 9.00 | 3 | 120 | 52 | 175 |
| < 1.18 | 0 | 1 | 0 | 1 |
| Negative | 0 | 0 | 1 | 1 |
| Total | 3 | 124 | 53 | 180 |
- Citation: Qiu N, Li R, Yu JG, Yang W, Zhang W, An Y, Li T, Liu XE, Zhuang H. Comparison of Abbott and Da-an real-time PCR for quantitating serum HBV DNA. World J Gastroenterol 2014; 20(33): 11762-11769
- URL: https://www.wjgnet.com/1007-9327/full/v20/i33/11762.htm
- DOI: https://dx.doi.org/10.3748/wjg.v20.i33.11762