Original Article
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World J Gastroenterol. Sep 7, 2014; 20(33): 11762-11769
Published online Sep 7, 2014. doi: 10.3748/wjg.v20.i33.11762
Comparison of Abbott and Da-an real-time PCR for quantitating serum HBV DNA
Ning Qiu, Rui Li, Jian-Guo Yu, Wen Yang, Wei Zhang, Yong An, Tong Li, Xue-En Liu, Hui Zhuang
Ning Qiu, Rui Li, Tong Li, Xue-En Liu, Hui Zhuang, Department of Microbiology and Infectious Disease Center, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China
Jian-Guo Yu, Wen Yang, Wei Zhang, Yong An, Department of Internal Medicine, Liver Disease Center, No. 88 Hospital of People’s Liberation Army, Taian 27100, Shandong Province, China
Author contributions: Qiu N and Li R performed the majority of experiments; Yu JG, Yang W, Zhang W and An Y managed the clinical therapy and collected serum samples; Qiu N and Liu XE performed the analysis and interpretation of data; Liu XE designed this comparative study and was involved in writing and editing the manuscript; Li T and Zhuang H revised the manuscript critically for important content.
Supported by A Major Science and Technology Special Project of China Twelfth Five-year Plan, No. 2013ZX10002004 and No. 2012ZX10002003
Correspondence to: Xue-En Liu, MD, Associate Professor of Microbiology, Department of Microbiology and Infectious Disease Center, School of Basic Medical Sciences, Peking University Health Science Center, No. 38 Xueyuan Road, Haidian District, Beijing 100191, China. xueenliu@bjmu.edu.cn
Telephone: +86-10-82802413 Fax: +86-10-82802413
Received: December 2, 2013
Revised: March 27, 2014
Accepted: April 21, 2014
Published online: September 7, 2014
Core Tip

Core tip: The hepatitis B virus (HBV) DNA values measured by the Abbott or Da-an real-time polymerase chain reaction assay were significantly correlated with the expected values of HBV DNA standards. A Bland-Altman plot showed good agreement between the assays. For clinical evaluation, HBV DNA levels derived from the Abbott assay were significantly higher than those of the Da-an assay.