Intestinal stem cell marker LGR5 expression during gastric carcinogenesis

Table 1 LGR5 immunostaining in gastric cancer-related gastric tissues and metastases n (%)

Pathological type	Total, n	LGR5 expression		P value
		Negative	Positive
Normal tissue	145	106 (73.1)	39 (26.9)	0.000
Dysplasia grade	53	26 (49.1)	27 (50.9)
Low	25	8 (32.0)	17 (68.0)	0.019
High	28	18 (64.3)	10 (35.7)
TNM stage	180	85 (47.2)	95 (52.8)
I-II	71	27 (38.0)	44 (62.0)
III-IV	109	58 (53.2)	51 (46.8)
Metastases	15	4 (26.7)	11 (73.3)
Lymph node	5	1 (20.0)	4 (80.0)
Liver	10	3 (30.0)	7 (70.0)

LGR5: Leucine-rich repeat-containing G protein-coupled receptor 5; TNM: Tumor, nodes, metastasis.

Table 2 Association of immunodetected LGR5 expression with clinicopathological features of gastric cancer patients n (%)

Clinicopathological feature	LGR5 expression		P value
	Negative	Positive
Sex			0.072
Male	56 (43.1)	74 (56.9)
Female	29 (58.0)	21 (42.0)
Age, yr			0.005
≤ 60	48 (58.5)	34 (41.5)
> 60	37 (37.8)	61 (62.2)
Location in stomach			0.657
Upper	15 (40.5)	22 (59.5)
Mid	17 (47.2)	19 (52.8)
Lower	45 (49.5)	46 (50.5)
Lesion size in cm			0.612
≤ 4	33 (42.9)	44 (57.1)
> 4	32 (47.1)	36 (52.9)
Differentiation			0.006
Differentiated	31 (36.5)	54 (63.5)
Undifferentiated	54 (56.8)	41 (43.2)
Histological type			0.579
Adenocarcinoma	67 (46.2)	78 (53.8)
Others	18 (51.4)	17 (48.6)
Lauren type			0.035
Intestinal	48 (41.4)	68 (58.6)
Diffuse/other	37 (57.8)	28 (42.2)
Lymphovascular invasion			0.288
No	43 (43.9)	55 (56.1)
Yes	42 (51.9)	39 (48.1)
Depth			0.833
T1-T2	19 (48.7)	20 (51.3)
T3-T4	66 (46.8)	75 (53.2)
Lymph node metastasis			0.934
No	19 (47.5)	21 (52.5)
Yes	65 (46.8)	74 (53.2)
Metastasis			0.160
No	71 (45.2)	86 (54.8)
Yes	14 (60.9)	9 (39.1)
TNM			0.046
I-II	27 (38.0)	44 (62.0)
III-IV	58 (53.2)	51 (46.8)

LGR5: Leucine-rich repeat-containing G protein-coupled receptor 5; TNM: Tumor, nodes, metastasis.

Table 3 LGR5 expression in gastric cancer tissues of various differentiation n (%)

Tissue	LGR5 expression		P value
	Negative	Positive
Intestinal metaplasia	25 (27.8)	65 (72.2)	0.000
Normal tissue	106 (73.1)	39 (26.9)
Dysplasia with IM			0.004
Yes	3 (18.8)	13 (81.2)
No	23 (62.2)	14 (37.8)
Lauren type			0.035
Intestinal	48 (41.4)	68 (58.6)
Diffuse/other	37 (57.8)	28 (42.2)
Intestinal type GC			0.019
Metastasis or recurrence	6 (12.5)	21 (31.3)
No metastasis or recurrence	42 (87.5)	46 (68.7)

LGR5: Leucine-rich repeat-containing G protein-coupled receptor 5; GC: Gastric cancer; IM: Intestinal metaplasia.