Protective effect of naringenin on acetic acid-induced ulcerative colitis in rats

Table 1 Effect of naringenin on microscopic scoring of histopathological sections of colonic tissue of rats with acetic acid-induced ulcerative colitis

Groups	Ulceration	Hyperemia	Necrosis	Edema	Cellular infiltrate	Goblet cell hyperplasia
Control	0	0	0	0	0	0
AA	+++	+++	++++	+++	++++	++
NG25 + AA	++	++	+++	++	+++	++
NG50 + AA	++	++	++	++	+++	+
NG100 + AA	+	+	+	+	++	+
MES + AA	+	+	+	+	+	+

MES: Mesalazine; AA: Acetic acid; NG: Naringenin; NG25: NG 25 mg/kg per day; NG50: 50 mg/kg per day; NG100: 100 mg/kg per day; 0: Normal; +: Mild; ++: Moderate; +++: Sever; ++++: Very sever.

Table 2 Effect of naringenin on DNA, RNA and total protein levels in colonic tissue of rats with acetic acid-induced ulcerative colitis

Groups	DNA (μg/100 mg wet tissue)	RNA (μg/100 mg wet tissue)	Total protein (mg/100 mg wet tissue)
Control	652.05 ± 17.12b	378.51 ± 38.44b	3.0 ± 0.19b
NG100	688.84 ± 47.69	380.24 ± 48.96	2.43 ± 0.31
AA	222.69 ± 18.78d	167.35 ± 15.16d	0.95 ± 0.08c
NG25 + AA	293.93 ± 33.49	208.68 ± 25.05	1.32 ± 0.13
NG50 + AA	338.54 ± 21.44	250.06 ± 10.11	2.02 ± 0.46
NG100 + AA	415.50 ± 41.51	298.34 ± 12.92	2.15 ± 0.27
MES + AA	425.04 ± 38.23	307.44 ± 18.31	2.14 ± 0.26

Values are expressed as mean ± SE (n = 6) and analyzed using one way ANOVA followed by Newman-Keuls post hoc test.

^bP < 0.01 control vs acetic acid (AA);

^cP < 0.05,

^dP < 0.01 AA vs Naringenin (NG) 25 + AA, NG50 + AA, NG100 + AA or mesalazine (MES) + AA groups.