Effects of SAHA on proliferation and apoptosis of hepatocellular carcinoma cells and hepatitis B virus replication

Table 1 Inhibition rate of suberoylanilide hydroxamic acid on HepG2.2.15 cells

SAHA (μmol/L)	24 h	48 h	72 h
2.5	8% ± 0.54%	15% ± 1.52%	23% ± 1.39%
5.0	13% ± 0.63%	22% ± 1.68%	34% ± 1.61%
7.5	28% ± 1.56%	39% ± 1.67%	50% ± 1.70%
10.0	42% ± 1.72%	51% ± 1.82%	66% ± 1.76%

P < 0.05 for comparison among different concentration and different treatment time, respectively. SAHA: Suberoylanilide hydroxamic acid.

Table 2 Effect of suberoylanilide hydroxamic acid on apoptosis rate of HepG2.2.15 cells

Group	24 h		48 h
	Early apoptosis rate	Middle-late apoptosis rate	Early apoptosis rate	Middle-late apoptosis rate
Control	3.25%	1.08%	3.58%	1.26%
2.5 μmol/L SAHA	16.28%	5.16%	23.06%	8.42%
5.0 μmol/L SAHA	21.02%	10.70%	26.44%	17.55%

SAHA: Suberoylanilide hydroxamic acid.

Table 3 Effect of suberoylanilide hydroxamic acid on proportion of HepG2.2.15 cells with different phases

Group	24 h			48 h
	G0/G1	S	G2/M	G0/G1	S	G2/M
Control	50.3%	34.9%	14.8%	46.3%	38.2%	15.5%
2.5 μmol/L SAHA	69.9%	22.3%	7.8%	70.9%	26.1%	3.0%
5.0 μmol/L SAHA	65.3%	20.6%	14.1%	68.9%	25.8%	5.3%

SAHA: Suberoylanilide hydroxamic acid.