miR-20b, miR-98, miR-125b-1*, and let-7e* as new potential diagnostic biomarkers in ulcerative colitis

Table 1 Patients’ characteristics (validation cohort)

Characteristics	Control (n = 20)	Inactive UC (n = 19)	Active UC (n = 20)
Gender (male/female)	10/10	6/13	9/11
Age (yr), mean (range)	48 (24-83)	48 (21-69)	38 (16-79)
Age at diagnosis (< 25/> 25 yr)	-	4/15	7/13
Years with disease (< 10/> 10 yr)	-	8/11	15/5
Mayo score, mean (range)	-	0 (0-1)	6 (2-12)
Extension of disease (P/PS/PC/LC/PH)	-		3/3/7/5/2
Smoking/non-smoking	5/15	6/13	3/17
EIM (present/never present)	-	3/16	0/20
Daily medications, n (%)
Systemic mesalazine (1.6-3.2 mg)	-	14 (74)	17 (85)
Topical mesalazine (1000 mg)	-	3 (16)	6 (30)
Systemic glucocorticoids (75 mg)	-	0 (0)	2 (10)
Topical glucocorticoids (100 mg)	-	0 (0)	2 (10)
Azathioprine (100-200 mg)	-	1 (5)	2 (10)
6-mercaptopurine (50-100 mg)	-	0 (0)	1 (5)
Infliximab (5 mg/kg)	-	0 (0)	1 (5)
None	20 (100)	5 (26)	2 (10)

EIM: Extraintestinal manifestations; LC: Left-sided colitis; P: Proctitis; PC: Pancolitis; PH: Proctitis hemorrhagica; PS: Proctosigmoiditis; UC: Ulcerative colitis.

Table 2 Top fifty differentially expressed miRNAs (active ulcerative colitis vs inactive ulcerative colitis, active Crohn’s disease, inactive Crohn’s disease, and controls) and their respective fold-changes from miRNA microarray expression profiling

MicroRNAs	Active UC/active CD	Active UC/controls	Active UC/inactive CD	Active UC/inactive UC
hsa-miR-15a	64	7	19	24
hsa-miR-199b-3p	55	6	17	61
hsa-miR-20b	53	12	20	35
hsa-miR-20a	51	9	14	47
hsa-miR-106b	43	4	7	40
hsa-miR-27b	36	6	6	15
hsa-miR-99a	33	33	17	31
hsa-miR-222	32	7	12	26
hsa-miR-151-5p	19	4	18	20
hsa-miR-203	19	11	17	24
hsa-miR-30a	14	9	14	7
hsa-miR-25	14	6	9	11
hsa-miR-26b	12	15	10	13
hsa-miR-646	12	19	13	8
hsa-miR-100	10	4	3	10
hsa-miR-125b	10	15	8	4
hsa-miR-98	10	10	10	12
hsa-miR-411*	8	3	8	5
hsa-miR-28-5p	8	3	3	6
hsa-miR-768-3p	7	5	4	17
hsa-miR-195	7	10	35	22
hsa-miR-99b	7	4	4	3
hsa-miR-23a	7	8	18	49
hsa-miR-18a	7	11	7	8
hsa-miR-17	7	5	6	44
hsa-miR-155	6	7	11	19
hsa-miR-23b	6	7	10	18
hsa-miR-1201	6	6	6	8
hsa-miR-130a	6	6	11	26
hsa-miR-199a-3p	6	5	15	37
hsa-miR-93	5	3	4	8
hsa-miR-199a-5p	5	3	3	7
hsa-miR-16	5	8	17	175
hsa-miR-146a	5	3	3	6
hsa-miR-103	5	3	6	30
hsa-miR-126	5	16	39	65
hsa-miR-107	5	3	7	18
hsa-miR-106a	5	5	6	21
hsa-miR-1248	4	5	8	93
hsa-miR-27a	4	6	6	25
hsa-miR-222*	4	4	4	6
hsa-miR-24	3	3	4	17
hsa-miR-182	3	3	3	5
hsa-miR-193a-3p	3	7	3	7
hsa-let-7e	3	2	4	2
hsa-miR-548a-3p	2	2	2	6
hsa-miR-99a*	1	1	1	3
hsa-miR-758	1	1	1	2
hsa-miR-568	1	5	6	7

Differentially up-regulated (≥ 10-fold) miRNAs are indicated in bold in the lists. *miRNA complementary strands. CD: Crohn’s disease; UC: Ulcerative colitis.

Table 3 Top fifty differentially expressed miRNAs (inactive ulcerative colitis vs active ulcerative colitis, active Crohn’s disease, inactive Crohn’s disease, and controls) and their respective fold-changes from miRNA microarray expression profiling

MicroRNAs	Active UC/inactive UC	Inactive UC/controls	Active CD/inactive UC	Inactive UC/inactive CD
hsa-miR-506	13	4	5	5
hsa-miR-125b-1*	12	6	27	5
hsa-let-7e*	6	6	6	6
hsa-miR-512-5p	5	5	5	4
hsa-miR-637	5	3	4	4
hsa-miR-1288	4	4	4	5
hsa-miR-330-3p	4	5	9	4
hsa-miR-623	4	6	6	6
hsa-miR-34b	4	9	9	7
hsa-miR-138-1*	3	6	6	5
hsa-miR-154	3	7	7	7
hsa-miR-760	3	3	17	3
hsa-miR-1296	3	5	11	4
hsa-miR-523	3	3	3	3
hsa-miR-149	3	5	10	5
hsa-miR-509-3-5p	3	3	3	3
hsa-miR-1276	3	3	19	4
hsa-miR-1178	3	4	2	5
hsa-miR-885-5p	3	3	3	3
hsa-miR-1264	2	2	2	2
hsa-miR-521	2	6	6	5
hsa-miR-218-2*	2	3	2	2
hsa-miR-551a	2	8	13	4
hsa-miR-505*	2	2	3	2
hsa-miR-1226	2	3	15	4
hsa-miR-495	2	4	9	3
hsa-miR-220c	2	3	3	4
hsa-miR-550*	2	4	2	3
hsa-miR-371-3p	2	3	3	3
hsa-miR-596	2	4	4	3
hsa-miR-216a	2	6	9	4
hsa-miR-1293	2	3	15	3
hsa-miR-1247	2	3	47	2
hsa-miR-34c-3p	2	4	12	5
hsa-miR-1233	2	3	6	3
hsa-miR-346	2	3	4	3
hsa-miR-211	2	8	9	11
hsa-miR-302c*	2	5	11	6
hsa-miR-520a-3p	2	2	2	2
hsa-miR-485-3p	2	5	14	3
hsa-miR-92a-2*	2	2	4	3
hsa-miR-328	1	2	2	2
hsa-miR-661	1	3	7	4
hsa-miR-453	1	7	8	5
hsa-miR-30c	1	5	7	7
hsa-miR-520h	1	2	2	2
hsa-miR-193b	1	4	10	4
hsa-miR-483-3p	1	2	4	2
hsa-miR-150	1	3	6	2

Differentially up-regulated (≥ 5-fold) miRNAs are indicated in bold in the lists. *miRNA complementary strands. CD: Crohn’s disease; UC: Ulcerative colitis.