Metabonomic studies of pancreatic cancer response to radiotherapy in a mouse xenograft model using magnetic resonance spectroscopy and principal components analysis

Table 1 Relative integrals and their ratios from some selected metabolites contributing to the classification of normal pancreas and pancreatic tumor tissues

		Normal pancreas	Pancreatic tumor	P-value
Metabolites	Choline	2.75 ± 1.37	3.99 ± 0.35	0.0376
	Taurine	1.99 ± 0.55	13.63 ± 2.92	0.0001
	Betaine	2.91 ± 0.57	1.58 ± 0.47	0.0002
	Glutamic acid	0.29 ± 0.11	0.46 ± 0.13	0.0260
	Alanine	0.60 ± 0.14	1.93 ± 0.16	0.0001
	Lactate	1.93 ± 0.86	8.30 ± 1.02	0.0001
	Acetic acid	0.06 ± 0.10	0.06 ± 0.03	0.7942
	Glycerophosphocholine+ phosphocholine	19.47 ± 1.36	16.61 ± 1.31	0.0007
Metabolites ratio	Glycerophosphocholine/Creatine	3.51 ± 0.76	2.35 ± 0.58	0.0042
	Phosphocholine/Creatine	5.19 ± 0.96	6.22 ± 1.52	0.1284

Table 2 Relative integrals and their ratios from some selected metabolites contributing to the evaluation of pancreatic tumor tissues response to radiotherapy

		Untreated	10 Gy	20 Gy	30 Gy	P-value
Metabolites	Choline	3.99 ± 0.35	3.97 ± 0.43	3.77 ± 0.36	3.44 ± 0.36	0.00751
						0.37402
						0.90123
	Taurine	13.63 ± 2.92	13.43 ± 3.25	11.45 ± 2.20	12.41 ± 3.03	0.42621
						0.11412
						0.90053
	Betaine	1.58 ± 0.47	1.69 ± 0.38	1.23 ± 0.45	0.79 ± 0.30	0.00131
						0.14662
						0.62753
	Glutamic acid	0.46 ± 0.13	0.38 ± 0.07	0.43 ± 0.10	0.48 ± 0.17	0.84081
						0.64802
						0.13663
	Alanine	1.93 ± 0.16	2.10 ± 0.40	2.01 ± 0.27	1.96 ± 0.42	0.88181
						0.48212
						0.28903
	Lactate	8.30 ± 1.02	7.79 ± 1.43	7.51 ± 1.33	7.55 ± 0.85	0.13161
						0.20312
						0.42593
	Acetic acid	0.06 ± 0.03	0.15 ± 0.06	0.25 ± 0.07	0.27 ± 0.13	0.00251
						0.00012
						0.00133
	Glycerophosphocholine + phosphocholine	16.61 ± 1.31	19.95 ± 5.87	20.59 ± 5.79	20.80 ± 5.44	0.05221
						0.07832
						0.13833
Metabolites ratio	Glycerophosphocholine/Creatine	2.35 ± 0.58	2.19 ± 0.15	2.49 ± 0.83	2.11 ± 0.36	0.33121
						0.70872
						0.44873
	Phosphocholine/Creatine	6.22 ± 1.52	5.92 ± 0.44	5.87 ± 1.09	6.51 ± 1.28	0.68051
						0.60652
						0.60963

¹Untreated vs 30 Gy;

²Untreatment vs 20 Gy;

³Untreatment vs 10 Gy.