Roles of BN52021 in platelet-activating factor pathway in inflammatory MS1 cells

doi:10.3748/wjg.v19.i25.3969

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Jul 7, 2013; 19(25): 3969-3979
Published online Jul 7, 2013. doi: 10.3748/wjg.v19.i25.3969

Table 1 Specific primers for Adcy1, Pla2g4a, Plcb3, Ptk7, Adrbk1, Mapk14 and Gapdh genes

Gene	Primer	Length (bp)	Annealing temperature (°C)
Adcy1
Forward	5'-GACTTTGTTCTCCGAGTTG-3'	19	49
Reverse	5'-GTGCTATCCATCCGACTG-3'
Pla2g4a
Forward	5'-GAATAAAGGCTCTACAATGG-3'	20	49
Reverse	5'-GTTGTCGCTTTGGTACTC-3'
Plcb3
Forward	5'-CCTCAACTTCAACCGAGTT-3'	19	49
Reverse	5'-CAGAGTGAGGTACGGCTTG-3'
Ptk7
Forward	5'-CACTGCGATGTCACATTG-3'	18	49
Reverse	5'-CACTATGTTCGGGACTGG-3'
Adrbk1
Forward	5'-AAGCCAGCCAACATTCTC-3'	18	51
Reverse	5'-CCCTTCTGTAGGACTTCG-3'
Mapk14
Forward	5'-GGACCTGAACAACATCGTG-3'	19	50
Reverse	5'-CTAGGTTGCTGGGCTTTAG-3'
Gapdh
Forward	5'-CATCTTCCAGGAGCGAGAC-3'	19	50
Reverse	5'-GGCTAAGCAGTTGGTGGTG-3'

Adcy1: Adenylate cyclase; Pla2g4a: Phospholipase A2; Plcb3: Phospholipase Cβ; Ptk7: Protein tyrosine kinase; Adrbk1: G protein-coupled receptor kinases; Mapk14: p38-mitogen-activated protein kinase; Gapdh: Glyceraldehyde 3-phosphate dehydrogenase.

Citation: Xia SH, Xiang XH, Chen K, Xu W. Roles of BN52021 in platelet-activating factor pathway in inflammatory MS1 cells. World J Gastroenterol 2013; 19(25): 3969-3979
URL: https://www.wjgnet.com/1007-9327/full/v19/i25/3969.htm
DOI: https://dx.doi.org/10.3748/wjg.v19.i25.3969