Wohl P, Hucl T, Drastich P, Kamenar D, Spicak J, Honsova E, Sticova E, Lodererova A, Matous J, Hill M, Wohl P, Kucera M. Epithelial markers of colorectal carcinogenesis in ulcerative colitis and primary sclerosing cholangitis. World J Gastroenterol 2013; 19(14): 2234-2241 [PMID: 23599650 DOI: 10.3748/wjg.v19.i14.2234]
Corresponding Author of This Article
Pavel Wohl, MD, Department of Hepatogastroenterology, Institute for Clinical and Experimental Medicine, Videnska 1958, 14021 Prague 4, Czech Republic. pawo@ikem.cz
Research Domain of This Article
Medicine, Research & Experimental
Article-Type of This Article
Brief Article
Open-Access Policy of This Article
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World J Gastroenterol. Apr 14, 2013; 19(14): 2234-2241 Published online Apr 14, 2013. doi: 10.3748/wjg.v19.i14.2234
Table 1 Demographic features of participating patients
n
Age (yr)
Sex (M/F)
Duration of UC (yr)
PSC
Duration after OLT (yr)
Histology
Endoscopy score (Mayo)
N
10
52.2 ± 14.09
4/6
0
No
0
0
0
UCR
7
41.57 ± 13.35
3/4
9.57 ± 1.59
No
0
0.42 ± 0.4
0.71 ± 0.45
UCA
9
45.88 ± 17.62
5/4
9.56 ± 2.41
No
0
2.7 ± 0.4
2.5 ± 0.49
PSC-UC
8
37.12 ± 6.8
5/3
8.75 ± 1.56
Yes (n = 8)
0
2.1 ± 0.59
1.12 ± 0.33
OLT
22
43.33 ± 12.11
11/11
12.4 ± 5.24
No
5.19 ± 2.61
1.4 ± 0.49
1.09 ± 0.29
Table 2 Monoclonal and polyclonal antibodies used in this study
Specificity
Origin
Company
Antigen retrieval
Dilution of antibodies
bcl-2 Oncoprotein
Mouse
DakoCytomation, Denmark
Buffer EDTA, pH 8
20 ×
p53
Mouse
DakoCytomation, Denmark
Tris/EDTA, pH 9
40 ×
COX-2
Mouse
Cayman, Michigan, United States
Citrate buffer, pH 6
40 ×
Table 3 Relationship between p53, cyclooxygenase-2 and primary sclerosing cholangitis n (%)
PSC-
PSC+
Row total
COX-2-
COX-2+
Row total
p53-
43 (78.18)
0 (0)
43 (78.18)
34 (61.82)
5 (9.09)
39 (70.91)
p53+
4 (7.27)
8 (14.55)
12 (21.82)
9 (16.36)
7 (12.73)
16 (29.09)
Column total
47 (85.45)
8 (14.55)
55 (100)
43 (78.18)
12 (21.82)
55 (100)
Table 4 Comparison of primary sclerosing cholangitis-ulcerative colitis and ulcerative colitis after liver transplantation for primary sclerosing cholangitis
Citation: Wohl P, Hucl T, Drastich P, Kamenar D, Spicak J, Honsova E, Sticova E, Lodererova A, Matous J, Hill M, Wohl P, Kucera M. Epithelial markers of colorectal carcinogenesis in ulcerative colitis and primary sclerosing cholangitis. World J Gastroenterol 2013; 19(14): 2234-2241