Brief Article
Copyright ©2013 Baishideng Publishing Group Co.
World J Gastroenterol. Mar 14, 2013; 19(10): 1593-1601
Published online Mar 14, 2013. doi: 10.3748/wjg.v19.i10.1593
Table 1 Confirmation and comparison of selected messenger RNA array results with those of quantitative real time polymerase chain reaction for selected genes
Array
qRT-PCR
GeneFold changeP valueFold changeP value
Heme vs DHA-6 h time point
HMOX159.99.97 × 10-987.910-35
IL-820.503.92 × 10-513.70.016
ANXA17.181.87 × 10-532.30.015
BACH13.043.26 × 10-53.10.0019
Heme vs DHA-24 h time point
HSPB877.12.66 × 10-6920.010
ANXA145.75.98 × 10-61380.015
IL-833.24.19 × 10-531.70.016
HMOX125.62.50 × 10-527.61.33 × 10-7
BACH24.507.24 × 10-522.60.011
PNPLA3-6.481.74 × 10-4-6.654.59 × 10-7
Table 2 Summary of data analysis with use of ingenuity pathway
6 h
24 h
NameP valueRatioP valueRatio
Top canonical pathways
Nrf2-mediated oxidative stress response2.64 × 10-1128/193 (0.145)1.58 × 10-961/193 (0.316)
Protein ubiquitination pathway1.44 × 10-931/274 (0.133)3.26 × 10-875/274 (0.274)
Aldosterone signaling in epithelial cells8.31 × 10-821/170 (0.124)--
Glucocorticoid receptor signaling1.6 × 10-524/295 (0.081)--
P53 signaling1.75 × 10-310/96 (0.104)--
Biosynthesis of steroids--3.22 × 10-714/121 (0.116)
Propanoate metabolism--1.45 × 10-624/121 (0.198)
Urea cycle and metabolism of amino groups--5.8 × 10-616/78 (0.205)
Top toxicity lists
Nrf2-mediated oxidative stress response3.03 × 10-1130/237 (0.127)5.41 × 10-966/237 (0.278)
Renal necrosis/cell death1.05 × 10-425/314 (0.08)1.06 × 10-579/314 (0.252)
Liver necrosis/cell death1.79 × 10-314/166 (0.084)--
P53 signaling2.06 × 10-310/95 (0.105)1.54E × 10-430/95 (0.316)
Liver proliferation2.54 × 10-312/133 (0.09)--
Cholesterol biosynthesis--2.26 × 10-1316/16 (1)
FXR/RXR activation--5.84 × 10-426/86 (0.302)
Table 3 Summary of analysis with ingenuity pathway analytical tools
6 h
24 h
NameP valueNo. moleculesP valueNo. molecules
Hepatotoxicity
Liver proliferation6.71 × 10-4 - 6.26 ×10-1124.24× 10-3 - 1.63× 10-136
Liver necrosis/cell death2.08 × 10-3 - 3.77 × 10-115--
Liver damage3.31 × 10-3 - 1.25 × 10-112--
Liver hemorrhaging7.28 × 10-3 - 7.28 × 10-32--
Hepatocellular peroxisome proliferation3.57 × 10-2 - 3.57 × 10-21--
Liver cholestasis--1.11 × 10-5 - 6.55 × 10-132
Hepatocellular carcinoma--2.46 × 10-4 -2.46 × 10-454
Liver steatosis--2.59 × 10-3 - 1.00 × 10-034
Liver fibrosis--1.51 × 10-2 - 4.43 × 10-119
Nephrotoxicity
Renal necrosis/cell death7.22 × 10-5 - 4.21 × 10-1257.44 × 10-7 - 1.00E × 10-079
Kidney failure1.27 × 10-3 - 2.24 × 10-1102.64 × 10-2 - 6.55 × 10-126
Renal enlargement1.27 × 10-3 - 1.03 × 10-122.64 × 10-2 - 4.13 × 10-12
Renal damage3.09 × 10-2 - 5.83 × 10-14
Glomerular3.57 × 10-2 - 5.17 × 10-12
Renal tubule injury4.27 × 10-5 - 1.63 × 10-130
Renal proliferation2.17 × 10-3 - 4.83 × 10-124