Effects of warm ischemia time on biliary injury in rat liver transplantation

Table 1 Effect of different secondary warm ischemia times on vascular endothelial growth factor-A level (mean ± SD)

Group	6 h	24 h
I	183.44 ± 11.65	185.04 ± 10.11
II	185.32 ± 12.06	154.55 ± 11.77a
III	181.42 ± 9.97	142.32 ± 10.30ac
IV	177.93 ± 10.40	126.44 ± 16.03bce

^aP < 0.05 vs group I;

^bP < 0.01 vs group I;

^cP < 0.05 vs group II;

^eP < 0.05 vs group III.

Table 2 Effect of different secondary warm ischemia time on the number of bile ducts and blood vessels in portal area (mean ± SD)

	Time (h)	Group I	Group II	Group III	Group IV
Bile ducts	6	6.10 ± 0.74	3.40 ± 1.17a	2.80 ± 0.79a	2.60 ± 0.51a
	24		3.00 ± 1.15a	2.40 ± 0.74ac	1.85 ± 0.63ace
Blood vessels	6	5.50 ± 0.94	2.90 ± 0.74a	2.40 ± 0.88a	2.00 ± 0.84a
	24		2.40 ± 0.93a	1.67 ± 0.67ac	1.10 ± 0.83ace
Bile ducts with blood vessels	6	5.42 ± 1.35	2.20 ± 1.23a	1.60 ± 0.70a	1.40 ± 0.81a
	24		1.80 ± 0.53a	1.10 ± 0.67ac	0.40 ± 0.65ace
Bile ducts without blood vessels	6	0.65 ± 0.42	1.0 ± 0.67a	1.20 ± 0.70a	1.31 ± 0.92a
	24		1.12 ± 0.53a	1.40 ± 1.03ac	1.58 ± 0.61ace

^aP < 0.05 vs group I;

^cP < 0.05 vs group II;

^eP < 0.05 vs group III.

Table 3 Effect of different secondary warm ischemia times on cholangiocyte proliferation, apoptosis index of bile duct epithelial cells and bile duct injury severity score (mean ± SD)

Group	Cholangiocyte proliferation		Apoptosis index		Severity score
	6 h	24 h	6 h	24 h	6 h	24 h
I	25.81 ± 3.50	26.13 ± 2.60	0.87 ± 0.50	0.53 ± 0.60	0	0
II	17.38 ± 4.31a	16.91 ± 5.67a	5.83 ± 0.51	7.15 ± 0.62	2.6 ± 0.3	2.8 ± 0.2
III	14.36 ± 3.69ac	12.90 ± 2.48ac	7.57 ± 0.32c	8.98 ± 0.65c	3.7 ± 0.3c	3.8 ± 0.4c
IV	10.19 ± 0.49ce	9.01 ± 3.65ce	8.96 ± 0.67ce	9.92 ± 0.47c	4.8 ± 0.2ce	5.0 ± 0.3ce

^aP < 0.05 vs group I;

^cP < 0.05 vs group II;

^eP < 0.05 vs group III.