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©2012 Baishideng Publishing Group Co.
World J Gastroenterol. Jul 28, 2012; 18(28): 3696-3704
Published online Jul 28, 2012. doi: 10.3748/wjg.v18.i28.3696
Published online Jul 28, 2012. doi: 10.3748/wjg.v18.i28.3696
Table 1 Interleukin-17A receptor short hairpin RNA and random short hairpin RNA sequences
| Target sequence | Double strand DNA oligo sequence | |
| IL-17RA shRNA 1 | CAAGTCCAAGACCATCTTA | 5'-ccggcaCAAGTCCAAGACCATCTTAttcaagagaTAAGATGGTCTTGGACTTGtgtttttg-3' |
| 5'-aattcaaaaacaCAAGTCCAAGACCATCTTAtctcttgaaTAAGATGGTCTTGGACTTGtg-3' | ||
| IL-17RA shRNA 2 | CCAGCGATCCAATGTCACA | 5'-ccggcaCCAGCGATCCAATGTCACAttcaagagaTGTGACATTGGATCGCTGGtgtttttg-3' |
| 5'-aattcaaaaacaCCAGCGATCCAATGTCACAtctcttgaaTGTGACATTGGATCGCTGGtg-3' | ||
| IL-17RA shRNA 3 | CAGCAGCCATGAACATGAT | 5'-ccggcaCAGCAGCCATGAACATGATttcaagagaATCATGTTCATGGCTGCTGtgtttttg-3' |
| 5'-aattcaaaaacaCAGCAGCCATGAACATGATtctcttgaaATCATGTTCATGGCTGCTGtg-3' | ||
| IL-17RA shRNA 4 | GGAAGAAAGTGGAGTGGTA | 5'-ccgggaGGAAGAAAGTGGAGTGGTAttcaagagaTACCACTCCACTTTCTTCCtctttttg-3' |
| 5'-aattcaaaaagaGGAAGAAAGTGGAGTGGTAtctcttgaaTACCACTCCACTTTCTTCCtc-3' | ||
| Random shRNA | TTCTCCGAACGTGTCACGT | 5'-ccggTTCTCCGAACGTGTCACGTttcaagagaACGTGACACGTTCGGAGAAtttttg-3' |
| 5'-aattcaaaaaTTCTCCGAACGTGTCACGTtctcttgaaACGTGACACGTTCGGAGAA-3' |
- Citation: Zhang SC, Zheng YH, Yu PP, Min TH, Yu FX, Ye C, Xie YK, Zhang QY. Lentiviral vector-mediated down-regulation of IL-17A receptor in hepatic stellate cells results in decreased secretion of IL-6. World J Gastroenterol 2012; 18(28): 3696-3704
- URL: https://www.wjgnet.com/1007-9327/full/v18/i28/3696.htm
- DOI: https://dx.doi.org/10.3748/wjg.v18.i28.3696