Lipopolysaccharide induces and activates the Nalp3 inflammasome in the liver

Table 1 Real-time polymerase chain reaction primers

Target gene	Forward primer (5' → 3')	Reverse primer (5' → 3')
18S	GTA ACC CGT TGA ACC CCA TT	CCA TCC AAT CGG TAG TAG CG
TNF-α	GAA GTT CCC AAA TGG CCT CC	GTG AGG GTC TGG GCC ATA GA
IL-1β	TCT TTG AAG TTG ACG GAC CC	TGA GTG ATA CTG CCT GCC TG
IL-1RA	TCA GAT CTG CAC TCA ATG CC	CTG GTG TTT GAC CTG GGA GT
Caspase-1	AGA TGG CAC ATT TCC AGG AC	GAT CCT CCA GCA ACT TC
ASC	GA GCT GCT GAC AGT GCA AC	GCC ACA GCT CCA GAC TCT TC
Nalp3	AGC CTT CCA GGA TCC TCT TC	CTT GGG CAG TTT CTT TC
Nlrc4	TGG TGA CAA TAG GGC TCC TC	CTG TTC CCT TTG CTC ACC TC
Nalp1	TGG CAC ATC CTA GGG AAA TC	TCC TCA CGT GAC AGC AGA AC
Pannexin-1	TGT GGC TGC ACA AGT TCT TC	ACA GAC TCT GCC CCA CAT TC

TNF: Tumor necrosis factor; IL: Interleukin; ASC: Apoptosis-associated speck-like, caspase recruitment domain-domain containing protein.