Salvianolate inhibits cytokine gene expression in small intestine of cirrhotic rats

Table 1 Forward and reverse primers used in quantitative real-time polymerase chain reaction

Gene	Primer sequence (5’-3’)	Annealing temperature (°C)	Product size (bp)
β-actin	5’-ACTGCCGCATCCTCTTCCTC-3’ 5’-ACTCCTGCTTGCTGATCCACAT-3’	55	598
Rat TNF-α	5’-GGCAGGTCTACTTTGGAGTCATTGC-3’	58	318
	5’-ACATTCGGGGATCCAGTGAGCTCCG-3’
Rat IL-6	5’-GGATACCACCCACAACAG-3’ 5’-GGTCCTTAGCCACTCCTT-3’	58	451

TNF: Tumor necrosis factor; IL: Interleukin.

Table 2 Mortality of rats in different groups

Group	n	Mortality values
A	10	0 (0/10)a
B	14	37.5% (6/14)
C	14	21.42% (3/14)
D	14	21.42% (3/14)
E	13	0 (0/13)a

^aP < 0.05 vs non treatment group. A: Normal control group; B: Non treatment group; C: Low-dose salvianolate treatment group; D: Medium-dose salvianolate treatment group; E: High-dose salvianolate treatment group.

Table 3 Distribution of endotoxin levels in serum of different groups

Group	n	Dose (mg/kg)	Levels of endotoxin (pg/L)
			0-1	1-10	10-20	> 20	Mean rank
A	10	-	10	0	0	0	15.35b
B	14	-	2	2	3	7	55.71
C	14	12	8	4	2	0	47.54a
D	14	24	12	1	1	0	29.14b
E	13	48	12	1	0	0	14.57b

^aP < 0.05,

^bP < 0.01 vs non treatment group. A: normal control group; B: non treatment group; C: low-dose salvianolate treatment group; D: medium-dose salvianolate treatment group; E: high-dose salvianolate treatment group.