Discovery and validation of prognostic markers in gastric cancer by genome-wide expression profiling

Table 1 Clinicopathological characteristics of all patients

Variables	Cases	Training dataset(n = 39)	Validation dataset(n = 33)	P
Sex
Male	53	28	25	0.79
Female	19	11	8
Age (yr)
mean ± SE	72	60.9 ± 1.5	61.6 ± 1.3	0.74
Depth of wall invasion
T2	4	3	1	0.121
T3	56	32	24
T4	12	4	8
Differentiation
Well	7	5	2	0.181
Moderate	31	14	17
Poor	27	18	9
Undifferentiated2	7
Lymph node metastasis
Negative	16	10	6	0.57
Positive	56	29	27
Distance metastasis
M0	66	38	28	0.09
M1	6	1	5
TNM stages
I + II	17	12	5	0.301
III	33	18	15
IV	22	9	13

¹The multiple comparisons of different subclasses;

²Data was incomplete.

Table 2 The ten-gene prognostic marker correlated to patients’ survival

Symbol	Cox score	Description
RPS19	2.93	Ribosomal protein S19
RPS8	2.90	Ribosomal protein S8
RPS2P12	2.62	Predicted ribosomal protein S2
RPS12	2.59	Ribosomal protein S12
RPS15P4	2.57	Predicted 40S ribosomal protein S15
RPLP2	2.51	Ribosomal protein, large, P2
EIF3S6	2.61	Eukaryotic translation initiation factor 3
GLTSCR2	2.67	Tumor suppressor candidate region gene 2
TMSB10	2.47	Thymosin, beta 10
SEC61G	-2.98	Sec61 gamma subunit

Table 3 Association between different prognosis groups identified by the ten-gene marker and the clinicopathological characteristics

Variables	Cases	Prolonged survival(n = 12)	Poor survival(n = 21)	P
Sex
Male	14	9	5	0.070
Female	18	3	15
Age (yr)
mean ± SE	33	58.1 ± 2.2	63.5 ± 1.5	0.040
Depth of wall invasion
T2	1	1	0	0.120
T3	24	11	13
T4	8	0	8
Differentiation
Well	2	1	1	0.490
Moderate	17	8	9
Poor	9	2	7
Undifferentiated1	5
Lymph Node Metastasis
Negative	6	4	2	0.160
Positive	27	8	19
Distance metastasis
M0	28	12	16	0.080
M1	5	0	5
TNM stages
II	5	5	0	0.002
III	15	5	10
IV	13	2	11

¹Data was incomplete. P values for stage, grade and location of tumors were derived from the Pearson χ² test. P value for age was derived from the t test.

Table 4 Multivariate Cox regression for overall survival in validation dataset

Variables	P	HR	CI (95%)
Depth of wall invasion	0.370	1.66	0.55-4.90
Differentiation	0.240	4.93	0.15-1.58
Lymph node metastasis	0.780	0.77	0.13-4.53
Distance metastasis	0.120	0.32	0.08-1.35
Ten-gene prognostic marker	0.007	0.13	0.29-0.56

CI: Confidence interval; HR: Hazard ratio.