Oncolytic adenovirus SG600-IL24 selectively kills hepatocellular carcinoma cell lines

Table 1 Concentration of melanoma differentiation-associated-7/interleukin-24 protein in different hepatocellular carcinoma cell lines and normal liver cell line (pg/mL)

	Concentration of MDA-7/IL-24 protein
	HepG2	Hep3B	MHCC97L	L02
Control group	8.0 ± 1.0	9.0 ± 0.5	4.0 ± 0.4	9.0 ± 0.8
Ad.IL-24 group	8.2 ± 0.5	9.0 ± 1.0	4.5 ± 0.5	10.0 ± 1.0
SG600-EGFP group	9.0 ± 0.8	10.0 ± 0.1	3.5 ± 0.5	9.0 ± 0.6
SG600-IL24 group
24 h	90 ± 10b	60 ± 8a	56 ± 10b	110 ± 12b
48 h	160 ± 20b	90 ± 15b	180 ± 20b	180 ± 15b
72 h	780 ± 80b	800 ± 60b	680 ± 50b	920 ± 80b

^aP < 0.05 vs Ad.IL-24 and SG600-EGFP groups;

^bP < 0.01 vs control group. MDA: Melanoma differentiation-associated; IL: Interleukin.

Table 2 Hoechst staining and flow cytometry showing apoptosis of hepatocellular carcinoma cell lines and normal liver cell line (mean ± SD)

Cell line	Apoptotic cells (%)				F-value	P-value
	Control	Ad.IL-24	SG600-EGFP	SG600-IL24
Hoechst staining
HepG2	2.5 ± 0.1	4.0 ± 0.3	5.5 ± 0.3	42.0 ± 4.5	156.6	0.000007
Hep3B	3.0 ± 0.2	3.5 ± 0.3	4.5 ± 0.2	56.0 ± 3.8	202.4	0.000003
MHCC97L	1.6 ± 0.2	3.0 ± 0.4	2.6 ± 0.3	40.5 ± 1.9	143.2	0.000010
L02	1.0 ± 0.1	1.2 ± 0.2	1.4 ± 0.1	2.0 ± 0.1	1.78	0.360
Flow cytometry
HepG2	2.0 ± 0.1	4.2 ± 0.4	10.0 ± 2.0	56.5 ± 4.0	203.4	0.000003
Hep3B	2.5 ± 0.2	5.0 ± 0.2	13.5 ± 1.5	78.3 ± 3.5	313.2	0.000001
MHCC97L	1.4 ± 0.2	3.2 ± 0.4	10.4 ± 1.0	43.3 ± 2.5	160.6	0.000006
L02	1.0 ± 0.1	1.2 ± 0.1	1.4 ± 0.1	1.7 ± 0.1	1.62	0.280