Clinical significance of Helicobacter pylori cagA and iceA genotype status

Table 1 Polymerase chain reaction primers for amplification of cagA, iceA1 and iceA2 genes

Amplified gene	Primer destination	Sequence of primer	Size of PCR product (bp)	Ref.
cagA	D008 F	ATAATGCTAAATTAGACAACTTGAGCGA	297
	R008 R	TTAGAATAATCAACAAACATCACGCCAT		[4,5]
iceA1	iceA1 F	GTGTTTTTAACCAAAGTATC	247
	iceA1 R	CTATAGCCASTYTCTTTGCA		[6]
iceA2	iceA2 F	GTTGGGTATATCACAATTTAT	229 or 334	[1]
	iceA2 R	TTRCCCTATTTTCTAGTAGGT

PCR: Polymerase chain reaction; F: Forward primer; R: Reverse primer.

Table 2 Genotype and virulence factors in relation to clinical conditions n (%)

Clinical outcome	Starin genotypes			Total
	cagA	iceA1	iceA2
PUD	17 (51.5)	11 (33.3)	5 (15.1)	33 (33.3)
NUD	26 (39.3)	18 (27.2)	10 (15.1)	66 (66.6)
Total	43 (43.3)	29 (29.2)	15 (15.1)	99 (100)

n: No. of Helicobacter pylori positive strains with the given characteristics; PUD: Peptic ulcer disease; NUD: Non ulcer dyspepsia.

Table 3 Distribution of virulence factors cagA, iceA1 and iceA2 among different ethnic groups in the study sample n (%)

Ethnic group	Strain genotypes			Total
	cagA	iceA1	iceA2
Malays	12 (37)	5 (15)	2 (6)	32
Chinese	21 (48)	17 (39)	9 (20)	43
Indians	7 (38)	4 (22)	2 (11)	18
Others	3 (50)	3 (50)	2 (33)	6

n: Total No. of patients of the respective ethnicity.