Brief Article
Copyright ©2010 Baishideng.
World J Gastroenterol. Jul 28, 2010; 16(28): 3553-3560
Published online Jul 28, 2010. doi: 10.3748/wjg.v16.i28.3553
Table 1 Primer sequences, annealing temperature, and product sizes for methylation-specific polymerase chain reaction assays
CpG statusGenesForward primer (5’→3’)Reverse primer (5’→3’)Genomic position2Annealing temperature (°C)Product size (bp)
MhMLH1ACGTAGACGTTTTATTAGGGTCGCCCTCATCGTAACTACCCGCG-716 to -60255115
UTTTTGATGTAGATGTTTTATTAGGGTTGTACCACCTCATCATAACTACCCACA-721 to -59855124
MMGMT1TTTCGACGTTCGTAGGTTTTCGCGCACTCTTCCGAAAACGAAACG+142 to +22362121
UTTTGTGTTTTGATGTTTGTAGGTTTTTGTAACTCCACACTCTTCCAAAAACAAAACA+137 to +23062133
MCDKN2A/p16TTATTAGAGGGTGGGGCGGATCGCCCACCTAAATCGACCTCCGACCG+167 to +40165234
UTTATTAGAGGGTGGGGTGGATTGTCCACCTAAATCAACCTCCAACCA+167 to +40155234
Table 2 Promoter methylation profiles for hMLH1, CDKN2A/p16, and MGMT in the four patient groups n (%)
Patient grouphMLH1 (tissue)
CDKN2A/p16 (tissue)
MGMT (tissue)
MGMT (blood)
MUMUMUMU
G I (n = 20)6/20 (30)14/20 (70)3/20 (15)17/20 (85)9/20 (46)11/20 (54)8/20 (40)12/20 (60)
G II (n = 18)7/18 (39)11/18 (61)4/18 (22)14/18 (78)7/18 (39)11/18 (61)1/18 (6)17/18 (94)
P = 0.563P = 0.581P = 0.666P = 0.0191
G III (n = 21)10/21 (48)11/21 (52)14/21 (67)7/21 (33)16/21 (76)5/21 (24)7/17 (41)10/17 (59)
P = 0.576P = 0.0081P = 0.0251P = 0.0191
G IV (n = 20)13/20 (65)7/20 (35)14/20 (70)6/20 (30)18/20 (90)2/20 (10)11/18 (64)7/18 (36)
P = 0.279P = 0.837P = 0.242P = 0.245