Expression of calreticulin is associated with infiltration of T-cells in stage IIIB colon cancer

Table 1 Parameters of patients (n = 68)

Parameters	n (%)
Age (yr)
< 60	30 (44.1)
≥ 60	38 (55.9)
Gender
Male	38 (55.9)
Female	30 (44.1)
Tumor site
Left hemicolon	45 (66.2)
Right hemicolon	23 (33.8)
Pathological grade
G1	10 (14.7)
G2	51 (75.0)
G3	7 (10.3)
Survival time (mo)
≥ 60	52 (76.5)
< 60	16 (23.5)

Table 2 Relation between expression of calreticulin, infiltration of lymphocytes and parameters of patients (n = 68)

Parameters	Calreticulin expression		P
	(-)	(+)-(+++)
Gender			0.813
Male	15	23
Female	11	19
Age (yr)			0.204
≥ 60	12	26
< 60	14	16
Tumor site			0.245
Left hemicolon	15	30
Right hemicolon	11	12
Pathological grade			0.643
G1	5	5
G2	19	32
G3	2	5
Infiltration of CD3+ cells per high-power field			0.387
> 18	18	33
≤ 18	8	9
Infiltration of CD45RO+ cells per high-power field			0.010
> 26	15	36
≤ 26	11	6

Table 3 Univariate survival analysis (n = 68)

Parameters	Survival time (mo)		P
	<60	≥ 60
Gender			0.542
Male	10	28
Female	6	24
Age (yr)			0.973
≥ 60	9	29
< 60	7	23
Tumor sites			0.722
Left hemicolon	10	35
Right hemicolon	6	17
Pathological grade			0.919
G1	2	8
G2	12	39
G3	2	5
Infiltration of CD3+ cells per high-power field			0.000
> 18	6	45
≤ 18	10	7
Infiltration of CD45RO+ cells per high-power field			0.000
> 26	5	46
≤ 26	11	6
Calreticulin expression
(-)	10	16
(+)-(+++)	6	36	0.022

Table 4 Multivariate survival analysis (n = 68)

Parameters	B	SE	Wald	df	Sig.	Exp(B)	95% CI for Exp (B)
							Lower	Upper
Pathological grade	0.172	0.261	0.435	1	0.510	1.188	0.712	1.979
Gender	-0.027	0.247	0.012	1	0.912	0.973	0.599	1.580
Age	0.079	0.255	0.095	1	0.758	1.082	0.656	1.784
Tumor site	0.085	0.276	0.094	1	0.759	0.919	0.535	1.579
Infiltration of CD3+ cells	-0.057	0.511	0.013	1	0.911	0.944	0.347	2.537
Infiltration of CD45RO+ cells	-0.743	0.552	1.809	1	0.179	0.476	0.161	1.404
Expression level of calreticulin	0.133	0.279	0.226	1	0.634	0.876	0.507	1.512

B: Regression coefficient; SE: Standard error; df: degree of freedom; Sig.: Significant; Exp (B): Odds ratio; CI: Confidence interval.