Establishment of a human hepatoma multidrug resistant cell line in vitro

doi:10.3748/wjg.v16.i18.2291

Advanced Search

BPG is committed to discovery and dissemination of knowledge

Home / Archive / Volume 16, Issue 18

This Article

Citation of this article

Corresponding Author of This Article

Article-Type of This Article

Open-Access Policy of This Article

Times Cited Counts in Google of This Article

Number of Hits and Downloads for This Article

Total Article Views (4596)

All Articles published online

The chart showing PDF series, HTML series, Figures (1-5) series, Tables (1-3) series.

Item

Count

PDF

626

HTML

3642

Figures (1-5)

189

Tables (1-3)

139

Sum=4596

May 14, 2010 (publication date) through Apr 25, 2024

Times Cited of This Article

Times Cited (23)

Journal Information of This Article

Publication Name

World Journal of Gastroenterology

ISSN

1007-9327

Publisher of This Article

Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Brief Article

World J Gastroenterol. May 14, 2010; 16(18): 2291-2297
Published online May 14, 2010. doi: 10.3748/wjg.v16.i18.2291

Table 1 IC₅₀ and RI values of SK-Hep-1 and SK-Hep-1/CDDP cells

Drug (μg/mL)	IC₅₀ (mean ± SD)		RI
Drug (μg/mL)	SK-Hep-1	SK-Hep-1/CDDP	RI
CDDP	5.13 ± 0.09	70.61 ± 1.06^b	13.76
DOX	0.74 ± 0.04	4.13 ± 0.23^b	5.58
VCR	0.76 ± 0.02	2.28 ± 1.06^b	3.12
5-FU	12.49 ± 0.27	52.79 ± 3.85^b	4.23

^bSignificant difference in comparison with the parental cells (P < 0.01). SK-Hep-1 and SK-Hep-1/CDDP cells were exposed to indicated concentrations of CDDP, DOX, VCR and 5-FU for 24 h and determined using the CCK-8 assay. The IC₅₀ values were calculated. Values of 3 independent experiments are represented as mean ± SD. Resistance index (RI) = (IC₅₀ SK-Hep-1/CDDP cells)/(IC₅₀ SK-Hep-1 cells). CDDP: Cisplatin; IC₅₀: 50% inhibitory dose; DOX: Doxorubicin; VCR: Vincristine; 5-FU: 5-fluorouracil.

Table 2 Cell cycle distribution of SK-Hep-1 and SK-Hep-1/CDDP cells (%)

Cell	G0/G1	S	G2/M
SK-Hep-1	59.83 ± 3.28	27.91 ± 2.16	12.14 ± 3.36
SK-Hep-1/CDDP	37.50 ± 5.05^a	42.20 ± 2.65^a	20.67 ± 5.69^a

^aP < 0.05, resistant cells vs SK-Hep-1. SK-Hep-1/CDDP in G0/G1 phase decreased accompanied by an increased proportion of cells in the S phase and G2/M phase.

Table 3 Semi-quantification of P-gp and MRP1 immunofluorescence (mean ± SD)

Cell	P-gp (n = 3)	MRP1 (n = 3)
SK-Hep-1	20.57 ± 6.59	3.75 ± 0.02
SK-Hep-1/CDDP	64.79 ± 12.4^a	35.78 ± 6.17^a

^aP < 0.05, resistant cells vs SK-Hep-1.

Citation: Zhou Y, Ling XL, Li SW, Li XQ, Yan B. Establishment of a human hepatoma multidrug resistant cell line in vitro. World J Gastroenterol 2010; 16(18): 2291-2297
URL: https://www.wjgnet.com/1007-9327/full/v16/i18/2291.htm
DOI: https://dx.doi.org/10.3748/wjg.v16.i18.2291