Suppression of matrix metalloproteinase-2 via RNA interference inhibits pancreatic carcinoma cell invasiveness and adhesion

Table 1 Down-regulation of MMP-2 mRNA expression detected by RT-PCR

Groups	Ratio of MMP-2 to GAPDH
Blank control	0.93 ± 0.02
pGPU6 (-)	0.91 ± 0.03
pGPU6-1	0.29 ± 0.02bd
pGPU6-2	0.33 ± 0.04bd
pGPU6-3	0.45 ± 0.03bd
pGPU6-4	0.88 ± 0.03ac

^aP > 0.05 vs blank control, P = 0.057;

^bP < 0.05 vs blank control;

^cP > 0.05 vs pGPU6 (-), P = 0.288;

^dP < 0.05 vs pGPU6 (-).

Table 2 Down-regulation of MMP-2 protein expression detected by Western blot

Groups	Relative density
Blank control	0.86 ± 0.03
pGPU6 (-)	0.82 ± 0.02
pGPU6-1	0.18 ± 0.02bd
pGPU6-2	0.31 ± 0.04bd
pGPU6-3	0.41 ± 0.02bd
pGPU6-4	0.82 ± 0.03ac

^aP > 0.05 vs blank control, P = 0.102;

^bP < 0.05 vs blank control;

^cP > 0.05 vs pGPU6 (-), P = 0.885;

^dP < 0.05 vs pGPU6 (-).

Table 3 Effects of MMP-2 siRNA on tumor cell adhesion

Groups	A value
Blank control	0.35 ± 0.03
pGPU6 (-)	0.34 ± 0.04
pGPU6-1	0.13 ± 0.02bd
pGPU6-2	0.20 ± 0.01bd
pGPU6-3	0.26 ± 0.01bd
pGPU6-4	0.33 ± 0.02ac

^aP > 0.05 vs blank control, P = 0.119;

^bP < 0.05 vs blank control;

^cP > 0.05 vs pGPU6 (-), P = 0.274;

^dP < 0.05 vs pGPU6 (-).

Table 4 Effects of MMP-2 siRNA on tumor cell invasion

Groups	Invasive cell number
Blank control	180 ± 12
pGPU6 (-)	176 ± 8
pGPU6-1	42 ± 7bd
pGPU6-2	49 ± 9bd
pGPU6-3	58 ± 6bd
pGPU6-4	171 ± 10ac

^aP > 0.05 vs blank control, P = 0.090;

^bP < 0.05 vs blank control;

^cP > 0.05 vs pGPU6 (-), P = 0.328;

^dP < 0.05 vs pGPU6 (-).