Gao QJ, Liu DW, Zhang SY, Jia M, Wang LM, Wu LH, Wang SY, Tong LX. Polymorphisms of some cytokines and chronic hepatitis B and C virus infection. World J Gastroenterol 2009; 15(44): 5610-5619 [PMID: 19938203 DOI: 10.3748/wjg.15.5610]
Corresponding Author of This Article
Dian-Wu Liu, MD, Department of Epidemiology, Public Health College, Hebei Medical University, Shijiazhuang 050017, Hebei Province, China. liudw56@tom.com
Article-Type of This Article
Brief Article
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World J Gastroenterol. Nov 28, 2009; 15(44): 5610-5619 Published online Nov 28, 2009. doi: 10.3748/wjg.15.5610
Table 1 The position, polymorphism and primer sequence in IL-2, IL-4 and IL-10 genes
SNP position
Polymorphism
Primer sequence 5’-3’
PCR conditions
IL-2 -330
T→G
F: 5'-TATTCACATGTTCAGTGTAGTTCT-3'
94°C, 48°C, 72°C for 1 min
R: 5'-ACATTAGCCCACACTTAGGT-3'
IL-4 -589
C→T
F: 5'-ACTAGGCCTCACCTGATACG-3'
94°C, 57°C, 72°C for 30 s
R: 5'-GTTGTAATGCAGTCCTCCTG-3'
IL-10 -592
A→C
F: 5'-CCTAGGTCACAGTGACGTGG-3'
94°C/30 s, 60°C/45 s, 72°C/60 s
R: 5'-GGTGAGCACTACCTGACTAGC-3'
Table 2 Polymorphism position, product size, restriction enzyme, digestion fragments in IL-2-330, IL-4-589 and IL-10-592
SNP position
Product size (bp)
Restriction enzyme
Digestion fragments (bp)
IL-2-330 T/G
150
MaeI (Bio Basic Inc.)
150
26 + 124
IL-4-589 T/C
252
BsmFI (New England BioLabs)
252
60 + 192
IL-10-592 C/A
412
RsaI (Bio Basic Inc.)
412
236 + 176
Table 3 HWE test for cytokines
Locus
Observed homozygosity (%)
Expected homozygosity (%)
P value
Wild-type genotype
Mutant genotype
Wild-type genotype
Mutant genotype
IL-2-330
40.79
14.08
40.14
13.43
> 0.05
IFN-γ+874
11.55
35.02
14.65
38.10
> 0.05
IL-4-589
3.25
50.54
6.94
54.24
> 0.05
IL-10-592
42.60
10.83
43.40
11.64
> 0.05
IL-10-1082
1.08
35.02
10.91
44.85
< 0.05
Table 4 Polymorphisms of IL-2-330, IFN-γ+874, IL-10-1082 and -592 and IL-4-589 and HBV and HCV infection n (%)
Genotype allele
Controls (n = 74)
HCV infection (n = 55)
HBV infection (n = 69)
HBV/HCV coinfection (n = 79)
χ2
P
IL-2-330
TT
22 (29.7)
24 (43.6)
33 (47.8)
34 (43.0)
GG
19 (25.7)
6 (10.9)
4 (5.8)
10 (12.7)
14.24
0.03
TG
33 (44.6)
25 (45.5)
32 (46.4)
35 (44.3)
T
77 (52.0)
73 (66.4)
98 (71.0)
103 (65.2)
12.33
0.01
G
71 (48.0)
37 (33.6)
40 (29.0)
55 (34.8)
IFN-γ+874
TT
7 (9.5)
5 (9.1)
9 (13.0)
11 (13.9)
AA
14 (18.9)
23 (41.8)
25 (36.2)
35 (44.3)
16.15
0.01
TA
53 (71.6)
27 (49.1)
35 (50.7)
33 (41.8)
T
67 (45.3)
37 (33.6)
53 (38.4)
55 (34.8)
4.87
0.18
A
81 (54.7)
73 (66.4)
85 (61.6)
103 (65.2)
IL-10-1082
GG
1 (1.4)
2 (3.6)
0 (0.0)
0 (0.0)
AA
16 (21.6)
21 (38.2)
27 (39.1)
33 (41.8)
13.05
0.04
GA
57 (77.0)
32 (58.2)
42 (60.9)
46 (58.2)
G
59 (39.9)
36 (32.7)
42 (30.4)
46 (29.1)
4.65
0.20
A
89 (60.1)
74 (67.3)
96 (69.6)
112 (70.9)
IL-10-592
AA
34 (45.9)
20 (36.4)
29 (42.0)
35 (44.3)
CC
9 (12.2)
6 (10.9)
9 (13.0)
6 (7.6)
2.83
0.83
AC
31 (41.9)
29 (52.7)
31 (44.9)
38 (48.1)
A
99 (66.9)
69 (62.7)
89 (64.5)
108 (68.4)
1.10
0.78
C
49 (33.1)
41 (37.3)
49 (35.5)
50 (31.6)
IL-4-589
CC
5 (6.8)
1 (1.8)
1 (1.4)
2 (2.5)
TT
38 (51.4)
28 (50.9)
35 (50.7)
39 (49.4)
4.41
0.62
CT
31 (41.9)
26 (47.3)
33 (47.8)
38 (48.1)
C
41 (27.7)
28 (25.5)
35 (25.4)
42 (26.6)
0.26
0.97
T
107 (72.3)
82 (74.5)
103 (74.6)
116 (73.4)
Table 5 Polymorphisms of IL-2-330, IFN-γ+874, IL-4-589, IL-10-1082 and -592 and clinical outcome n (%)
Genotype
Control (n = 74)
Mild CH (n = 122)
Moderate/severe CH (n = 57)
Cirrhosis (n = 24)
χ2
P
IL-2-330
TT
22 (29.7)
54 (44.3)
23 (40.4)
13 (54.2)
GG
19 (25.7)
14 (11.4)
6 (10.5)
1 (4.2)
13.46
0.04
TG
33 (44.6)
54 (44.3)
28 (49.1)
10 (41.7)
T
77 (52.0)
163 (66.8)
74 (64.9)
36 (75.0)
11.69
0.01
G
71 (48.0)
81 (33.2)
40 (35.0)
12 (25.0)
IFN-γ+874
TT
8 (10.8)
14 (11.5)
5 (8.8)
4 (16.7)
AA
14 (18.9)
48 (39.3)
26 (45.6)
9 (37.5)
14.78
0.02
TA
52 (70.3)
60 (49.2)
26 (45.6)
11 (45.8)
T
68 (45.9)
90 (36.9)
36 (31.6)
19 (39.6)
5.68
0.13
A
80 (54.1)
154 (63.1)
78 (68.4)
29 (60.4)
IL-10-1082
GG
1 (1.4)
1 (0.8)
1 (1.8)
0 (0.0)
AA
16 (21.6)
44 (36.1)
24 (42.1)
13 (54.2)
11.40
0.07
GA
57 (77.0)
77 (63.1)
32 (56.1)
11 (45.8)
G
59 (39.9)
79 (32.4)
34 (29.8)
11 (22.9)
5.85
0.12
A
89 (60.1)
165 (67.6)
80 (70.2)
37 (77.1)
IL-10-592
AA
34 (45.9)
51 (41.8)
22 (38.6)
11 (45.8)
CC
9 (12.2)
11 (9.0)
8 (14.0)
2 (8.3)
2.50
0.87
AC
31 (41.9)
60 (49.2)
27 (47.4)
11 (45.8)
A
98 (66.2)
163 (66.8)
71 (62.3)
33 (68.8)
0.92
0.82
C
50 (33.8)
81 (33.2)
43 (37.7)
15 (31.3)
IL-4-589
CC
3 (4.0)
3 (2.4)
3 (5.3)
0 (0.0)
TT
38 (51.4)
69 (54.8)
24 (42.1)
11 (45.8)
4.58
0.60
CT
33 (44.6)
54 (42.9)
30 (52.6)
13 (54.2)
C
39 (26.4)
58 (23.8)
36 (31.6)
13 (27.1)
2.46
0.48
T
109 (73.6)
186 (76.2)
78 (68.4)
35 (72.9)
Table 6 Polymorphisms of IL-2-330, IFN-γ+874, IL-4 -589 and IL-10-1082 and -592 and HCV RNA and ALT level n (%)
HCV RNA -
HCV RNA +
χ2
P
ALT < 80 U/L
ALT ≥ 80 U/L
χ2
P
IL-2-330
TT
29 (37.2)
39 (37.1)
102 (41.8)
11 (33.3)
GG
14 (17.9)
14 (13.3)
0.83
0.66
35 (14.3)
4 (12.1)
1.35
0.51
TG
35 (44.9)
52 (49.5)
107 (43.9)
18 (54.5)
T
93 (59.6)
130 (61.9)
0.20
0.66
311 (63.7)
40 (60.0)
0.24
0.62
G
63 (40.4)
80 (38.1)
177 (36.3)
26 (39.4)
IFN-γ+874
TT
13 (16.7)
10 (9.5)
29 (11.9)
3 (9.1)
AA
28 (35.9)
45 (42.9)
2.36
0.31
85 (34.8)
12 (36.4)
0.23
0.89
TA
37 (47.4)
50 (47.6)
130 (53.3)
18 (54.5)
T
63 (40.4)
70 (33.3)
1.92
0.17
188 (38.5)
24 (36.4)
0.12
0.74
A
93 (59.6)
140 (66.7)
300 (61.5)
42 (63.6)
IL-10-1082
GG
1 (1.3)
2 (1.9)
3 (1.2)
0 (0.0)
AA
14 (17.9)
44 (41.9)
12.27
0.00
81 (33.2)
16 (48.5)
3.25
0.20
GA
63 (80.8)
59 (56.2)
160 (65.6)
17 (51.5)
G
91 (58.3)
147 (70.0)
5.36
0.02
166 (34.0)
17 (25.8)
1.79
0.18
A
65 (41.7)
63 (128)
322 (66.0)
49 (74.2)
IL-10-592
AA
41 (52.6)
44 (41.9)
110 (45.1)
8 (24.2)
CC
6 (7.7)
11 (10.5)
2.10
0.35
27 (11.1)
3 (9.1)
6.32
0.04
AC
31 (39.7)
50 (47.6)
107 (43.9)
22 (66.7)
A
113 (72.4)
138 (65.7)
1.88
0.17
327 (67.0)
38 (57.6)
2.30
0.09
C
43 (27.6)
72 (34.3)
161 (33.0)
28 (42.4)
IL-4-589
CC
3 (3.8)
5 (4.8)
6 (2.5)
3 (9.1)
TT
48 (61.5)
55 (52.4)
1.53
0.47
132 (54.1)
8 (24.2)
12.46
0.02
CT
27 (34.6)
45 (42.9)
106 (43.4)
22 (66.7)
C
33 (21.2)
55 (26.2)
1.24
0.27
118 (24.2)
28 (42.4)
9.97
0.00
T
123 (78.8)
155 (73.8)
370 (75.8)
38 (57.6)
Citation: Gao QJ, Liu DW, Zhang SY, Jia M, Wang LM, Wu LH, Wang SY, Tong LX. Polymorphisms of some cytokines and chronic hepatitis B and C virus infection. World J Gastroenterol 2009; 15(44): 5610-5619