Association between MDM2-SNP309 and hepatocellular carcinoma in Taiwanese population

Table 1 The demography of HCC patients and cancer-free healthy controls from Taiwan n (%)

Characteristics1	Cases (58)	Controls (138)	P value
Age (yr)
mean	65.90	40.20	< 0.0001
SD	10.14	15.24
Gender
Male	45 (77.6)	42 (30.2)	< 0.0001
Female	13 (22.4)	96 (69.8)
Hepatitis
Yes	49 (84.5)	10 (7.2)	< 0.0001
No	9 (15.5)	79 (57.25)
Unknown	0	49 (35.5)
HBV2
+	29	10	0.0002
-	28	40
HCV3
+	20	0	< 0.0001
-	37	50
Alcohol intake
Yes	26 (44.8)	81 (58.7)	0.0851
No	32 (55.2)	57 (41.3)
Cardiovascular diseases and/or disorders
Yes	23 (39.7)	26 (18.7)	0.0035
No	35 (59.6)	116 (81.3)
BMI	22.7 ± 3.13	22.59 ± 3.44	0.8341

¹Age and BMI (Body Mass Index) were determined by t test. Other parameters were analyzed by Fisher’s exact test; ^2,3Two HCC cases were infected with both HBV and HCV. HCC: Hepatocellular carcinoma.

Table 2 The risk evaluation of MDM2 SNP309 genotypic frequencies on the development of HCC n (%)

SNP309	Cases	Controls	OR (95% CI)1	P value
TT	11 (19)	35 (25.3)	1
TG	37 (63.8)	80 (58)	1.016 (0.152-6.8)	0.99
GG	10 (17.2)	23 (16.7)	1.265 (0.074-21.767)	0.87
TG+GG	47 (81)	103 (57.5)	1.037 (0.757-6.862)	0.97
TT+TG	48 (82.8)	115 (83.3)	1
GG	10 (17.2)	23 (16.7)	1.25 (0.123-12.66)	0.85

¹The multivariate logistic regression model was used to calculate odds ratio adjusted for gender, infection of hepatitis virus (B or C type), age, cardiovascular disease and/or diabetes.

Table 3 Estimation of odds ratio for different genotypes of MDM2-SNP309 in hepatitis viral infected patients with HCC n (%)

Hepatitis1	SNP309	Cases	Controls	OR2	95% CI	P value
Positive	TT	7 (14.3)	1 (10)	1.000
	TG	32 (65.3)	7 (70)	2.340	0.11-49.655	0.59
	GG	10 (20.4)	2 (20)	2.568	0.054-121.687	0.63
	TG+GG	42 (85.7)	9 (90)	2.376	0.115-48.896	0.57
Negative	TT	4 (44.4)	19 (24)	1.000
	TG	5 (55.6)	45 (57)	0.512	0.046-5.712	0.59
	GG3	0 (0)	15 (19)	-	-	-
	TG+GG	5 (55.6)	60 (76)	0.470	0.041-5.387	0.54

¹Include HBV or HCV infected patients with HCC;

²OR was adjusted for age, gender and cardiovascular diseases and/or diabetes;

³OR was not estimated because the number in case was zero.