Microsatellite alterations in phenotypically normal esophageal squamous epithelium and metaplasia-dysplasia-adenocarcinoma sequence

Table 1 Microsatellite alterations of un-diluted and diluted DNA in different esophageal tissues

Tissue DNA	Number studied	MSI			LOH
		Negative	At≥1 locus	At≥2 loci	Negative	At≥1 locus	At≥2 loci
Classification
SCC	23	12	5	6	17	3	3
ADC	18	11	4	31	15	2	12
Normal
Undilution	8	8	0	0	8	0	0
Dilution	8	3	2	33	4	2	24
Metaplasia
Undilution	8	7	1	0	7	1	0
Dilution	8	1	4	35	2	3	36
Dysplasia
Undilution	8	7	1	0	7	1	0
Dilution	8	1	3	47	2	2	48
Carcinoma
Undilution	8	6	2	0	6	2	0
Dilution	8	0	3	59	0	4	410

Normal: Normal esophageal squamous epithelium DNA; Metaplasia: Barrett esophageal columnar epithelium DNA; Dysplasia: Dysplastic esophageal epithelium DNA; Carcinoma: Esophageal adenocarcinoma DNA;

¹P = 0.758,

²P = 0.696,

³P = 0.026,

⁴P = 0.069,

⁵P = 0.009, ⁶P = 0.034,

⁷P = 0.009,

⁸P = 0.029,

⁹P = 0.004,

¹⁰P = 0.004 vs other groups.

Table 2 Microsatellite alterations of undiluted and diluted DNA at 7 loci in different esophageal tissues

Tissue DNA	Number studied	D2S123		D3S1616		D3S1300		BATRII		D5S346		D17S787		D18S61
		MSI	LOH	MSI	LOH	MSI	LOH	MSI	LOH	MSI	LOH	MSI	LOH	MSI	LOH
Classification
SCC	23	3	1	6	2	3	4	0	0	2	1	2	1	2	0
ADC	18	4	1	3	1	2	1	0	0	2	0	1	0	0	0
Normal
Undilution	8	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Dilution	8	2	1	1	2	2	1	0	0	1	0	1	1	1	1
Metaplasia
Undilution	8	0	0	1	1	0	0	0	0	0	0	0	0	0	0
Dilution	8	2	2	2	3	1	2	1	0	1	1	2	1	1	0
Dysplasia
Undilution	8	0	0	1	1	0	0	0	0	0	0	0	0	0	0
Dilution	8	4	3	4	3	4	3	1	0	1	1	4	2	1	1
Carcinoma
Undilution	8	1	1	1	1	0	0	0	0	0	0	0	0	0	0
Dilution	8	6	4	6	7	6	4	2	0	4	0	6	3	3	2

Normal: Normal esophageal squamous epithelium DNA; Metaplasia: Barrett esophageal columnar epithelium DNA; Dysplasia: Dysplastic esophageal epithelium DNA; Carcinoma: Esophageal adenocarcinoma DNA.