Heterozygous nucleotide-binding oligomerization domain-2 mutations affect monocyte maturation in Crohn’s disease

Table 1 Sequence primers used for detection of NOD2 mutations

Primers	Sequence
Arg702W (forward)	5’-GGCGCCCCTGGAATTC-3’
Arg702W (reverse)	5’-CCTCACCCGGTGCAGC-3’
Gly908Arg (forward)	5’-CCCAGCTCCTCCCTCTTTC-3’
Gly908Arg (reverse)	5’-AAGTCTGTAATGTAAACGCCAC-3’
Leu1007fsinsC (forward)	5’-GAATGTCAGAATCAGAAGGG-3’
Leu1007fsinsC (reverse)	5’-GTCTCACCATTGTATCTTCTTTTC-3’

Table 2 Genotype and allelic frequencies for NOD2 variants in CD patients and controls

	CD (n = 47)	Healthy controls (n = 69)
Genotype
Hz	17/47 (36.1%)	4/69 (5.8%)
Double Hz	3/47 (6.4%)	0
mtNOD2	20/47	4/69
wtNOD2	27/47	0/69
Allelic frequencies
R702W	5.30%	1.45%
G908R	7.45%	0.75%
1007fs	8.50%	0.75%

Table 3 CDAI and drug therapy in mtNOD2 and wtNOD2 patients

	mtNOD2 (n = 20)	wtNOD2 (n = 27)
CDAI	24.5	25.2
Active disease	14	16
Steroids	7	9
Methotrexate	1	1
Azathioprine	4	5
Aminosalicylic acid	6	8
Salazopyrine	4	4
Infliximab	1	1
Thalidomide	4	5