Screening and cloning for proteins transactivated by the PS1TP5 protein of hepatitis B virus: A suppression subtractive hybridization study

Table 1 Quantitative analysis of mRNA

Transfectedplasmid	A230	A260	A280	A260/A280	mRNAquantitation(mg/L)
pcDNA3.1(-)-myc- his(A)-PS1TP5	0.173A	0.377A	0.172A	2.192	15.08
pcDNA3.1(-)-myc- his(A)	0.191A	0.378A	0.173A	2.185	14.84

Table 2 Homolog searching of sequenced cDNA fragments from SSH library

High similarity proteins to known genes	n	Homology (%)
Homo sapiens ribosomal protein S16 (RPS16)	2	98
Homo sapiens ribosomal protein LP0 (RPLP0)	1	98
Homo sapiens ribosomal protein LP2 (RPLP2)	1	100
Homo sapiens ribosomal protein S7 (RPS7)	1	100
Homo sapiens ribosomal protein L31 (RPL31)	1	100
Homo sapiens transmembrane 4 superfamily member 4-CD81 (TM4SF4)	1	99
Homo sapiens inositol monophosphatase 2 (IMPA2)	2	99
Homo sapiens protein kinase BRPK	1	100
Homo sapiens clusterin (CLU)	1	100
Homo sapiens adenosine deaminase	2	100
Homo sapiens solute carrier family 7 member 5 (SLC7A5)	1	99
Homo sapiens cytochrome c oxidase subunit 8A	2	100
Homo sapiens enolase 1	1	99
Homo sapiens H3 histone family 3B	1	100
Homo sapiens replication factor C	2	98
Human cytoskeletal gamma-actin gene	1	100
Homo sapiens S100 calcium-binding protein A6 (calcyclin)	1	99
Homo sapiens eukaryotic translation elongation factor 1	1	100
Homo sapiens epoxide hydrolase 1	1	98
Homo sapiens dehydrogenase 1	1	99
Homo sapiens signal sequence receptor-β	2	100
Homo sapiens β5-microtubulin	1	99
Homo sapiens pyruvate dehydrogenase	1	98
New genes with unknown function	1	100