H Pylori
Copyright ©2007 Baishideng Publishing Group Co.
World J Gastroenterol. Mar 14, 2007; 13(10): 1547-1553
Published online Mar 14, 2007. doi: 10.3748/wjg.v13.i10.1547
Table 1 Rates of immune protection of H pylori vaccine with different kinds of adjuvant
GroupsnH pylori positiven (%)Rates of immuneprotection (%)
(1) Control1010 (100)0
(2) Chi-solution1212 (100)0
(3) Chi-particles1313 (100)0
(4) Hp antigen1010 (100)0
(5) Hp antigen + CT125 (41.67)58.33d
(6) Hp antigen + chi-solution104 (40)60ab
(7) Hp antigen + chi-particles104 (40)60ab
P < 0.001
Table 2 H pylori colonization score in gastric mucosa
GroupsnH pylori colonization score
0123
(1) Control100244
(2) Chi-solutiond121821
(3) Chi-particlesd1301012
(4) Hp antigenc101522
(5) Hp antigen + CTad127212
(6) Hp antigen + chi-solutionb106400
(7) Hp antigen + chi-particlesb107300
H = 57.181, P < 0.001
Table 3 Density of H pylori colonization in gastric mucosa
GroupsnMedian of H pyloricolony number (CFU/g)
(1) Control104.74 × 105
(2) Chi-solution120.73 × 105
(3) Chi-particles131.83 × 105
(4) Hp antigen100.48 × 105
(5) Hp antigen + CT120
(6) Hp antigen + chi-solution100
(7) Hp antigen + chi-particles100
H = 27.43, P = 0.001
Table 4 Levels of IL-2, IFN-γ, IL-12, IL-10 and IL-4 in gastric mucosa before challenge (mean ± SD)
GroupsnIL-2IFN-γIL-12IL-10IL-4
(1) Control519.9 ± 12.628.6 ± 10.2118.9 ± 46.267.2 ± 32.54.19 ± 2.95
(2) Hp antigen520.6 ± 3.533.6 ± 13.7165.3 ± 47.4104.3 ± 19.16.49 ± 2.61
(3) Hp antigen chi-solution528.6 ± 9.358.5 ± 12.2b283.5 ± 93.7cd255.3 ± 131.8cd14.70 ± 8.73a
(4) Hp antigen+ chi-particles527.6 ± 13.959.8 ± 15.2b283.4 ± 99.6cd237.1 ± 98.3cd14.48 ± 6.84a
F = 0.739F = 7.948F = 6.083F = 6.228F = 4.189
P = 0.544P = 0.002P = 0.006P = 0.005P = 0.023
Table 5 Levels of IL-2, IFN-γ, IL-12, IL-10 and IL-4 in gastric mucosa after challenge (mean ± SD)
GroupsnIL-2IFN-γIL-12IL-10IL-4
(1) Control1034.3 ± 11.848.7 ± 27.8233.7 ± 125.786.4 ± 38.23.87 ± 1.99
(2) Chi-solution1131.8 ± 27.456.8 ± 26.1308.5 ± 178.7100.7 ± 52.64.47 ± 1.89
(3) Chi-particles1051.1 ± 42.658.8 ± 28.1311.3 ± 129.688.4 ± 51.77.21 ± 4.02g
(4) Hp antigen997.9 ± 64.2a59.4 ± 15.0319.4 ± 136.389.3 ± 29.014.89 ± 3.15
(5) Hp antigen + CT1080.9 ± 60.2c93.6 ± 23.5ef487.0 ± 289.3ef81.8 ± 49.53.67 ± 1.76
(6) Hp antigen + chi-solution10124.3 ± 75.2b107.5 ± 42.0d525.6 ± 112.2d108.5 ± 39.08.78 ± 4.96hi
(7) Hp antigen + chi-particles1088.6 ± 57.0a105.9 ± 48.1d554.0 ± 164.4d93.1 ± 39.26.59 ± 1.38k
F = 3.370F = 6.346F = 5.448F = 0.745F = 3.214
P = 0.002P < 0.001P < 0.001P > 0.05P = 0.003
Table 6 Levels of anti-HpIgG2a, IgG1 and ratio of IgG2a/IgG1 in serum after challenge (mean ± SD)
GroupsNIgG2aIgG1IgG2a/IgG1
(1) Control104.44 ± 2.092.77 ± 1.182.13 ± 1.24
(2) Chi-solution116.28 ± 3.623.96 ± 2.321.64 ± 0.85
(3) Chi-particles105.56 ± 2.274.57 ± 2.091.43 ± 0.37gh
(4) Hp antigen99.21 ± 6.80a6.43 ± 4.88a2.356 ± 1
(5) Hp antigen + CT1011.22 ± 5.52a7.71 ± 5.11de2.19 ± 0.86
(6) Hp antigen + chi-solution1011.48 ± 5.55a9.04 ± 5.35b1.06 ± 0.4f
(7) Hp antigen + chi-particles1016.13 ± 10.20b8.02 ± 5.29cd1 ± 0.37f
F = 4.572F = 10.308F = 3.780
P < 0.001P < 0.001P < 0.001